Apelin-13对在体大鼠心肌缺血-再灌注损伤的影响及其信号转导途径  

Effects of Apelin-13 on rat myocardial ischemia-reperfusion injury in vivo and its signal transduction pathway

作　　者：李卉[1] 曲新凯[1] 杨栓锁[1] 方唯一[1] 

机构地区：[1]上海交通大学胸科医院心内科,上海200030

出　　处：《上海交通大学学报（医学版）》2009年第6期637-640,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究心血管活性肽Apelin-13对在体大鼠心肌缺血-再灌注损伤的影响,并探讨其信号转导途径。方法将雄性SD大鼠随机分为对照组(n=17)和Apelin-13组(n=15),建立在体大鼠心肌缺血-再灌注模型,对照组在再灌注前5 min给予生理盐水颈静脉注射,Apelin-13组给予0.1 mg/kg Apelin-13颈静脉注射,用伊文思蓝和TTC双重染色法测定心肌梗死区(IS)面积和缺血危险区(ARR)面积,计算IS面积占ARR面积的百分比(IS/ARR);TUNEL法测定心肌细胞凋亡;Western blotting法检测ERK1/2蛋白的表达。结果Apelin-13组的IS/AAR和心肌细胞凋亡指数均显著低于对照组[(38.33±12.95)%vs(52.61±11.00)%,(0.21±0.02 vs 0.31±0.05](P<0.05);Apelin-13组p-ERK1/2的表达较对照组明显增加(1.15±0.16 vs 0.63±0.07)(P<0.05)。结论Apelin-13具有心肌保护作用,能够缩小心肌梗死面积,减少心肌细胞凋亡,其作用机制可能与ERK1/2 MAPK激酶途径激活有关。Objective To investigate the role of apelin-13, a vasoactive peptide, in rat myocardial ischemia-reperfusion injury in vivo and explore its signal transduction pathway. Methods Rats were randomly divided into control group （ n = 10） and Apelin-13 group （n = 15）, and in vivo models of rat myocardial ischemia-reperfusion injury were established. Normal saline （control group） or Apelin-13 （Apelin-13 group） was administered intravenously 5 min before reperfusion. TTC and Evan＇s blue staining were used to determine the infarction size （IS） and area at risk （AAR）, apoptotic cells were quantified by TUNEL method, and the expression of ERK1/2 was determined by Western blotting. Results IS/AAR and apoptosis index of Apelin-13 group were significantly lower than those in control group [ （38.33 ± 12.95）% vs （52.61 ± 11.00）% and （0.21 ± 0.02） vs （0.31 ± 0.05）] （P 〈 0.05）. The expression of p-ERK1/2 in Apelin-13 group was significantly increased than that in control group [ （ 1.15 ± 0.16） vs （0.63 ± 0.07） ] （P 〈 0.05）. Conclusion Apelin-13 may protect rat hearts from in vivo ischemia-reperfusion injury, reduce infarction size and attenuate myocardial apoptosis, which may be mediated by the activation of ERK1/2 MAPK signal transduction pathway.

关 键 词：心肌梗死 缺血-再灌注损伤 APELIN-13 细胞凋亡 

分 类 号：R542.22[医药卫生—心血管疾病] R332[医药卫生—内科学]