不育症患者精子凋亡率与精浆活性氧水平检测及相关性分析  

Detection and correlation analysis of sperm apoptosis rate and level of reactive oxygen species in seminal plasma among infertile males

作　　者：吕逸清[1] 陈斌[1] 胡凯[1] 向祖琼[1] 王益鑫[1] 黄翼然[1] 

机构地区：[1]上海交通大学医学院仁济医院泌尿外科上海市男科学研究所,上海200001

出　　处：《上海交通大学学报（医学版）》2009年第6期705-708,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市人口和计划生育委员会资助项目(2007JG06)~~

摘　　要：目的检测不育症患者精子凋亡率与精浆活性氧水平并分析其相关性。方法92例男性不育症患者,分为精索静脉曲张(VC)组(n=32)、白细胞精液症组(n=30)和其他原因组(n=30);另取24例成功进行体外授精精液样本作为对照组。采用计算机辅助精液分析系统行精液常规检测(精液pH值、精子密度和精子活力),Annexin V/PI染色法检测精子凋亡率,TBA法检测精浆活性氧水平。比较不育症各组与对照组各精液参数的差异,分析各组精子凋亡率与精浆活性氧水平的相关性。结果不育症各组精子活力显著低于对照组(P<0.01),VC组和白细胞精液症组精子凋亡率和精浆活性氧水平显著高于对照组(P<0.05或P<0.01)。白细胞精液症组精浆活性氧水平与精子凋亡率呈显著正相关(r=0.573,P<0.05)。结论精子凋亡率和精浆活性氧水平升高可能与VC患者和白细胞精液症患者不育相关,白细胞精液症患者活性氧水平升高可能是引起精子凋亡率升高的重要因素之一。Objective To detect the sperm apoptosis rate and level of reactive oxygen species （ROS） in seminal plasma and explore their correlation among infertile males. Methods Ninety-two inferitile males were divided into varicocele （VC） group （ n = 32）, leukocytospermia group（ n = 30） and the other cause group （ n = 30）, and another 24 in vitro fertilization sperm samples were sereved as controls. The routine sperm parameters including seminal pH, sperm viability and sperm density were examined by computer assisted sperm analysis, the sperm apoptosis rate was asseseed using Annexin V/PI staining, and the ROS level in seminal plasma was detected by TBA method. The differences in seminal parameters between three infertile groups and control group were compared, and the correlation of sperm apoptosis rate with level of ROS in seminal plasma was explored in each group. Results The sperm viability of three infertile groups was significantly lowerthan that of control group （P 〈 0.01）. The sperm apoptosis rates and levels of ROS in seminal plasma in VC group and leukocytospermia group were significantly higher than those in control group （P 〈 0.05 or P 〈 0.01）. The sperm apoptosis rate was positively correlated with the level of ROS in seminal plasma in leukocytospermia group（ r = 0. 573, P 〈 0.05）. Conclusion The increased sperm apoptosis rate and level of seminal plasma ROS may be related to the infertility of patients with VC and leukocytospermia. The increased level of seminal plasma ROS may be one of the causes of increased sperm apoptosis rate in patients with leukocytospermia.

关 键 词：不育 精子凋亡率 活性氧 精索静脉曲张 白细胞精液症 

分 类 号：R698.2[医药卫生—泌尿科学]