DENV2海南分离株NS1全序列测定和生物信息学分析  

The Gene Sequence Determination and Bioinformatic Analysis of the Nonstructure Protein 1 of Dengue 2 Virus Strain Isolated in Hainan

作　　者：周俊梅[1] 方丹云[1] 梁瑜[1] 尹悦[1] 江丽芳[1] 

机构地区：[1]中山大学中山医学院微生物学教研室,广东广州510080

出　　处：《生物技术》2009年第3期1-3,共3页Biotechnology

基　　金：广东省自然科学基金团队项目(05200638;06201946);广东省自然科学基金项目(7001516)资助

摘　　要：目的:对登革病毒2型海南分离株NS1全序列进行测定和生物信息学分析,了解其系统进化和分子流行病学特征。方法:采用RT-PCR法扩增D2-Hainan全长NS1基因,将其克隆入载体pPIcZαB,测序,采用相关软件进行生物信息学分析。结果:获得D2-Hainan株NS1全长基因1056bp,其序列与登革病毒2型NGC株的同源性为95%。系统进化树分析显示该毒株与登革病毒2型China04株的亲缘关系最近。初步分析了NS1蛋白的氨基酸序列特征和二级结构。结论:登革病毒流行株存在地域性和复杂性,对D2-Hainan株NS1基因及其编码蛋白信息特征的了解,有助于进一步研究其基因特征与病毒毒力的关系。Objective： Determine and analyze the entire sequence of the NS1 gene of dengue 2 virus strain isolated in Hainan which is to understand the characteristic of its molecular evolution. Method：The nucleotides of NS1 of D2 - Hainan was amplified by RT- PCR and the sequence was determined by Sanger method. Then the gene and the encoding protein were analyzed by BLAST, MEGA, Clustal X, nnPredict, HNN, and DAS software. Result： It showed that the whole nueleotide sequence of D2- Hainan NS1 was 1 056bp and had the 95% homology with that of NGC strain. It was most closely to China04 strain with the homology 99.3%. Bioinformatics analysis proved there were 352 deduced amino acids of NS1 protein and it shared the twelve highly conserved eysteine sequence among flavivimses. Conclusion： By carrying out sequencing and bioinformaties of NS1, our research established foundation of further explore for the relationship between the NS1 gene character and dengue virus vimlence.

关 键 词：登革病毒 NS1基因 生物信息学 

分 类 号：Q785[生物学—分子生物学]