草鱼RBM cDNA克隆及序列分析  

Sequence Analysis and cDNA Molecular Cloning to RBM Gene of Grass Carp

作　　者：熊钢[1,2] 刘臻[2] 王晓清[1] 鲁双庆[2] 刘小青[2] 罗小华[1] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]长沙学院,湖南长沙410008

出　　处：《生物技术》2009年第3期3-6,共4页Biotechnology

基　　金：湖南省自然科学基金项目(06JJ200506);湖南省教育厅重点项目(08A007)资助

摘　　要：目的:克隆草鱼RNA剪切蛋白(RNAbinding motif protein-RBM)基因。方法:采用RT—PCR技术从草鱼性腺总RNA中克隆RBM基因,将其插入pBS-T载体上,经菌液PCR鉴定后进行测序并对测序结果进行生物信息分析。结果:获得草鱼RBM cDNA部分序列,大小为384bp,预测编码127个氨基酸残基;RBM基因进化与物种形态进化类似;不同物种间RBM基因一级结构保守性不高,但氨基酸序列保守性较高,且存在高度保守的氨基酸位点。结论:成功地克隆了草鱼RBM cDNA部分序列,为获RBM cDNA全长序列及后续研究提供理论基础。Objective： To obtain the fragment related to RBM gene of grass carp. Method： The RBM gene partial cDNA fragment was amplified by RT-PCR from total RNA of grass carp＇s gonad. The sequence of RBM gene had been analysed with informatics after pBS-T coloning. Result：Sequencing results showed that the gene was 384 bp in length,encoding a preprotein of 404 aa residues; The evolution tree built by kimura way from the RBM homology of grass carp and other auimals was consistent with that of traditional taxonomic results; Analysis by DNAMAN showed that RBM genes had lowly homology, RBM aa residues had highly homology. Conclusion： The fragment related to RBM gene of grass carp was obtained.

关 键 词：草鱼 RBM 基因克隆 序列分析 

分 类 号：Q523.1[生物学—生物化学] Q785