不同修饰对壳聚糖转基因效果的影响  

Efficiency of Different Modify of Chitosan on Gene Transfection

作　　者：李轶杰[1] 蔡文萍[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《生物技术》2009年第3期14-16,共3页Biotechnology

基　　金：国家自然科学基金项目(30860265);新疆生物资源基因工程重点实验室基金项目(No.XJDX0201-2005-02)资助

摘　　要：目的:探讨不同修饰的壳聚糖包裹的质粒(Chi-DNA)纳米复合物在口服发送中外源基因在消化道中的表达差异。方法:分别使用明胶、海藻酸钠、PEG及乙酰化修饰包裹含LacZ的质粒pCMVβ的壳聚糖纳米颗粒,通过口服发送后经X-gal染色检测目的基因在小鼠体内的表达。结果:修饰后的Chi-DNA纳米复合物都能抵抗胃酸的降解0.5h以上,其中PEG及乙酰化修饰的Chi-DNA纳米复合物在胃酸处理1h时仍有部分残留。X-gal染色显示,修饰后的Chi-DNA纳米复合物都有β半乳糖苷酶的表达,其中PEG、海藻酸钠修饰的Chi-DNA纳米复合物在鼠胃和小肠中表达量最高。结论:PEG、海藻酸钠修饰的Chi-DNA纳米复合物有望成为高效的基因治疗用非病毒口服发送系统。Objective： To investigate the efficiency of different modification of Chitosan - DNA nanoparticles on gene transfection by oral delivery. Method： Oral administration of DNA nanoparticles synthesized by complexing pCMVβ DNA with chitosan, which modified with gelatin, Sodium Alginate, PEG and acetylize respectively, resulted in transduced gene expression in the intestinal epithelium. The tissues were stained with X-Gal according to standard protocols. Result： Compared with different nanoparticles to resist mouse gastric juice showed that the mochfication of DNA nanoparticles had good stability and although mice fed ‘naked＇ DNA showed some background staining, mice fed the DNA nanoparticles modified with Sodium Alginate or PEG showed a higher level of gene expression in both the stomach and the small intestine than others. Conclusion： These suggested that DNA nanoparticles modified with Sodium Alginate or PEG should be a potential oral delivery non- viral vector systems in gene therapy.

关 键 词：壳聚糖 修饰 纳米复合物 基因转染 

分 类 号：R944[医药卫生—药剂学]