大鼠心脏停搏供体动脉化原位肝移植模型制作体会  

A surgical experience of hepatic rearterialization in orthotopic liver transplantation of rats from non-heart beating donors

作　　者：张宝良[1] 袁庆鑫[1] 刘彤[2] 

机构地区：[1]河北医科大学附属唐山工人医院普外腔镜科,唐山063000 [2]天津医科大学总医院普通外科,天津300052

出　　处：《肝胆胰外科杂志》2009年第3期177-179,共3页Journal of Hepatopancreatobiliary Surgery

摘　　要：目的建立一个稳定的大鼠心脏停搏供体动脉化原位肝移植模型。方法根据供肝获取前经历供体心脏停搏时间的不同分为热缺血0min(W0组)、热缺血15min(W15组)和热缺血30min(W30组)3组,其后用Engemann法建立大鼠动脉化肝移植模型,比较术后肝功能、病理、手术成功率(>3d)和长期生存率(>100d)。结果W0、W15和W30组手术成功率分别为100.0%(15/15)、93.3%(14/15)和86.7%(13/15),术后长期生存率分别为100.0%(6/6)、83.3%(5/6)和66.7%(4/6),二者在3组间差异均无统计学意义。常规组织病理结果显示:随着供肝热缺血时间的延长,移植肝损伤加重。3组术后第3天血清丙氨酸转氨酶ALT和AST均无显著性改变。结论来自于无心跳供体的大鼠肝脏在热缺血30min以内是安全的,移植术后可以长期存活。该模型有助于肝移植热缺血损伤的研究。Objective To establish a reliable rats model of orthotopic liver transplantation with hepatic artery reconstruction from non-heart beating donors. Methods The model was established with Engemann＇s method. The rats were divided into three groups （W0, W15, W30, n=15/group） according to the cardiac arrest time from zero to 30 rain with 15 intervals. Hepatic function, pathology, the successful rate （longer than 3 days） of the model and the long-term survival （longer than 100 days） rate were compared. Results The successful rate and the long-term survival of W0, W15 and W30 were 100.0%（15/15）, 93.3%（14/15）, 86.7%（13/ 15） and 100.0%（6/6）, 83.3%（5/6）, 66.7%（4/6） respectively, which showed no significant difference in all three groups. Pathological studies showed liver parenchymal injury aggravated with the warm ischemia time. Serum levels of ALT and AST in all groups showed no marked difference on 3 days after surgery. Conclusion Rats liver graft can be safely subject to warm ischemia within 30 minutes and have long-term survival. The model is useful for the study of liver transplantation from warm ischemia of the graft.

关 键 词：肝移植 心脏停搏 大鼠 动物模型 

分 类 号：R657.3[医药卫生—外科学]