2种方法检测抗核抗体对系统性红斑狼疮诊断价值的比较  

Comparison of values of two tests of antinuclear antibody to diagnose SLE

作　　者：秦苏萍[1] 杜文平[1] 汤仁仙[1] 

机构地区：[1]徐州医学院病原生物学与免疫学教研室,江苏徐州221002

出　　处：《中国校医》2009年第3期264-265,共2页Chinese Journal of School Doctor

摘　　要：目的比较免疫金银法和免疫荧光法检测抗核抗体对系统性红斑狼疮(SLE)的诊断价值。方法对SLE和其余6种自身免疫性疾病患者及健康人血清采用免疫金银染色法和间接免疫荧光法检测抗核抗体,并比较分析两种方法检测抗核抗体对SLE诊断的敏感性、特异性、正确率、阳性预测值、阴性预测值、Youden指数等指标。结果免疫金银法和免疫荧光法检测抗核抗体阳性率为:SLE患者100%和96%、类风湿关节炎患者10%和36%、强直性脊柱炎患者2%和13%、系统性硬皮病患者13%和73%、干燥综合征患者27%和82%、皮肌炎患者12.5%和37.5%、血管炎患者0%和17%、健康人0%和0%。统计分析显示免疫金银染色法检测抗核抗体对SLE诊断的特异性、正确率、阳性预测值及Youden指数较免疫荧光法高,且差异有统计学意义(P<0.05)。结论免疫金银法检测ANA对SLE具有高度的敏感性和特异性,因此临床上采用免疫金银法检测ANA用于对SLE的辅助诊断更有价值。Objective To compared the values of two tests of antinuclear antibody to diagnose systemic lupus erythematosus （SLE）. Methods The sera from SLE cases, other 6 kinds of autoimmune disease cases and healthy controls were tested for the antinuclear antibody （ANA） by the immunogold-silver staining and immumofluorescence method. All the parameters were compared such as sensitivity, specificity, accuracy, positive predictive value, negative predictive value and Youden index. Results The ANA positive rates tested by the immunogold-silver staining and immumofluorescence method were 100% and 96% in SLE cases, 10% and 36% in rheumatoid arthritis eases, 2% and 13% in ankylosing spondylitis cases, 13% and 73% in dermatosclerosis cases, 27% and 82% in xero-syndrome cases, 12.5% and 37.5% in dermatomyositis cases, 0% and 17% in vaseulitis, and 0% and 0% in healthy controls, respectively. The specificity, accuracy, positive predictive value and Youden index of the immunogold-silver staining were higher than those of the immumofluorescence method, and the difference is significant, P ~ 0.05. Conehtsiorts The sensitivity and specificity of the immunogold-silver staining are high, so the immunogold-silver staining to test ANA is more valuable for SLE auxiliary diagnosis.

关 键 词：免疫金银法 荧光抗体技术 抗核抗体 免疫组织化学 红斑狼疮 系统性 

分 类 号：R593.241[医药卫生—内科学] R735.7[医药卫生—临床医学]