实验性精索静脉曲张对大鼠睾丸和附睾中血管内皮生长因子及其受体1表达的影响  

Experimental Varicocele Affects VEGF and Flt-1 Protein Expressions in the Testis and Epididymis of Adolescent Rats

作　　者：艾庆燕[1,2] 田宏[1] 马莉[2] 苗乃周[2] 霍涌玮[1] 王海旭[1] 王丽蓉[1] 邱曙东[1] 张秋养[1] 

机构地区：[1]西安交通大学医学院人体解剖与组织胚胎学系,陕西西安710061 [2]延安大学医学院组织胚胎学教研室,陕西延安716000

出　　处：《中华男科学杂志》2009年第6期488-492,共5页National Journal of Andrology

基　　金：国家自然科学基金项目(30371425,30471735);教育部科学技术重点研究资助项目(105157)~~

摘　　要：目的:研究实验性左侧精索静脉曲张(ELV)对青春期大鼠睾丸、附睾中血管内皮生长因子(VEGF)及其受体fms样酪氨酸激酶(Flt-1)蛋白表达的影响。方法:建立青春期雄性SD大鼠ELV模型,采用免疫组化SP法检测VEGF和Flt-1在ELV组及假手术对照组睾丸、附睾组织中的表达变化。结果:VEGF和Flt-1蛋白在ELV组和对照组大鼠睾丸、附睾中均有表达,其定位和表达量具有细胞特异性和区域特异性。经图像分析和统计检测显示,ELV2周和4周组双侧睾丸和附睾中VEGF蛋白的表达与相应对照组比较均显著增加(P<0.01和P<0.05);ELV2周组双侧睾丸和附睾中Flt-1蛋白的表达与相应对照组比较也显著增加(P<0.01和P<0.01),但4周时在双侧睾丸和左侧附睾中Flt-1蛋白的表达显著下降(P<0.01和P<0.05),而右侧附睾未见明显差异(P>0.05)。结论:实验性精索静脉曲张可造成青春期大鼠睾丸、附睾中VEGF和Flt-1的表达量变化,该变化可能会影响精子的发生和成熟,因而可能是VC引起男性生育力下降甚至不育的原因之一。Objective： To investigate the effects of experimental left varieoeele （ELV） on the vascular endothelial growth factor （VEGF） and it＇s receptor fins-like tyrosine kinase-1 （ Flt-1 ） proteins in the testis and epididymis of adolescent rats, and to find out the correlation of the two proteins with varicoeele-indueed male infertility. Methods ： We established the ELV model in adolescent male SD rats, and detected the expressions of VEGF and Flt-1 proteins in the testis and epididymis by immunohistochemistry at 2 and 4 weeks after surgery. Results ： Cell- and region-specific expressions of VEGF and Fit-1 were observed in the testis and epididymis of the ELV and control groups. Statistical analysis showed that, in comparison with the corresponding control groups, the 2- and 4-week ELV groups exhibited a notable increase in the VEGF protein expression in the hibateral testis and epididymis （P 〈 0.01, P 〈 0.05） ; the Flt-1 expression was obviously upregulated in the hibateral testis and epididymis of the 2-week ELV group （P 〈 0.01, P 〈 0.01 ）, but remarkably reduced in the hibateral testis and left epididymis of the 4-week ELV group （P 〈 0.01, P 〈 0.05 ） , with no statistic difference in the right epididymis （ P 〉 0.05 ）. Conclusion ： ELV can cause changes in the expressions of VEGF and Flt-1 proteins in the testis and epididymis of adolescent rats, and consequently affect spermatogenesis and spermiotelcosis, which may be one of the causes of varicocele-induced male infertility or subfertility.

关 键 词：实验性左侧精索静脉曲张 血管内皮生长因子 FMS样酪氨酸激酶 睾丸 附睾 免疫组化 

分 类 号：R697.24[医药卫生—泌尿科学]