CO释放抑制剂ZnPP Ⅸ和NO释放抑制剂L-NAME对大鼠阴茎组织cGMP含量影响的研究  

ZnPP Ⅸ and L-NAME Reduce the cGMP Content in the Penile Tissue of Rats

作　　者：马红斌[1] 秦文波[1] 桂士良[1] 

机构地区：[1]佳木斯大学附属第一医院泌尿外科,黑龙江佳木斯154002

出　　处：《中华男科学杂志》2009年第6期523-525,共3页National Journal of Andrology

基　　金：黑龙江省自然科学基金(D2005-08);黑龙江省卫生厅重点项目(2007-499)~~

摘　　要：目的:探讨CO释放抑制剂锌卟啉Ⅸ(ZnPPⅨ)和NO释放抑制剂N-硝基-L-精氨酸甲酯(L-NAME)对大鼠阴茎组织中cGMP含量的影响。方法:180～220g Wistar大鼠30只,随机均分为3组(正常对照组,ZnPPⅨ组,L-NAME组),ZnPPⅨ组给予ZnPPⅨ[45μmol/(kg.d)],L-NAME组给予L-NAME[50mg/(kg.d)],正常对照组给予生理盐水[1ml/(kg.d)]。用药7d后断头处死大鼠,取阴茎制成匀浆。测定匀浆液中NOS、NO、CO、cGMP含量。结果:与对照组比较,应用CO释放抑制剂ZnPPⅨ后,大鼠阴茎组织中CO、NOS、NO、cGMP含量有一定程度的下降(P<0.05);应用NO释放抑制剂L-NAME后,大鼠阴茎组织中CO、NOS、NO、cGMP含量也较对照组有一定程度的下降(P<0.05)。结论:ZnPPⅨ与L-NAME能够降低大鼠阴茎组织中CO与NO的浓度,同时cGMP的含量也降低。Objective ： To investigate the effects of CO release inhibitor zinc protoporphyria Ⅸ （ ZnPP Ⅸ ） and NO release inhibitor L- NAME on the content of cGMP in the penile tissue of rats. Methods ： Thirty Wistar rats were randomly divided into a normal control, a ZnPP Ⅸ, and an L-NAME group, given saline （1 ml/kg/d） , ZnPP Ⅸ （45 μmol/kg/d） and L-NAME （50 mg/kg/d）, respectively, for 7 days. Then all the rats were killed, homogenate made from their penile tissues and detected for the contents of NOS, NO, CO and cGMP. Results ： The contents of CO, NOS, NO and cGMP were all reduced in both the ZnPP Ⅸ and L-NAME groups as compared with the control group （ P 〈 0. 05 ）. Conclusion ： ZnPP Ⅸ and L-NAME can reduce the concentrations of CO and NO in the penile tissues of rats, and consequently the content of cGMP.

关 键 词：勃起功能障碍 锌卟啉Ⅸ N-硝基L-精氨酸甲酯 一氧化碳 一氧化氮合酶 一氧化氮 环磷酸鸟苷 阴茎 大鼠 

分 类 号：R697[医药卫生—泌尿科学]