乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒抑制HBsAg、HBVDNA分泌的体外实验  

Inhibition of hepatitis B surface antigen and hepatitis B virus DNA secretions by hepatitis B immunoglobulin poly （butylcynaoacrylate） nanoparticles in vitro

作　　者：彭忠田[1] 谭德明[1] 黄顺玲[1] 朱平安[1] 刘菲[1] 

机构地区：[1]中南大学湘雅医院传染病研究所,410008

出　　处：《中华传染病杂志》2009年第6期330-334,共5页Chinese Journal of Infectious Diseases

基　　金：湖南省长沙市科学技术局基金资助项目（K051155-72）

摘　　要：目的研究乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒（HBIGPBCA—NP）对HBV感染细胞模型HBsAg、HBVDNA分泌的抑制作用。方法用含HBIG-PBCA-NP及乙型肝炎免疫球蛋白（HBIG）的培养基培养HepG2．2．15细胞，或先以一定浓度的上述两种药物培养，第3天后更换为不含相应药物的培养基继续培养细胞，在不同时间点定量检测培养上清液HBsAg、HBVDNA。组间比较采用t检验和方差分析。结果0．1～10．0IU／mL的HBIGPBCA—NP及HBIG在不同时间点均能抑制HepG2．2．15细胞培养上清液HBsAg、HBVDNA的分泌，与对照组比较差异有统计学意义。HBIC-PBCANP组与HBIG组在无药物继续培养的上清液中，HBsAg浓度与HBVDNA水平在第5、7天继续下降，第9、11天逐步增高，其中0．1、1．0、5．0IU／mLHBIG-PBCA-NP组与同浓度HBIG组比较，培养上清液中的HBsAg浓度在第9天为（31．31±_1．98）μg／L比（40．62±2．99）μg／L、（23．79±1．31）μg／L比（36．51±2．12）μg／L、（19．91±1．74）μg/L比（33．03±1．65）μg／L（F=412．24，P〈0．01）。5．0IU／mL的HBIC-PBCA—NP组与同浓度的HBIG组比较，培养上清液中HBVDNA水平在第7天为（3．41±0．27）lg拷贝／mL比（6．56±0．19）lg拷贝／mL（t=14．75，P〈0．01）；第9天为（5．16±0．37）lg拷贝／mL比（6．87±0．30）lg拷贝／mL（￡=7．71，P〈0．01）；第11天为（6．40±0．33）lg拷贝／mL比（7．74±0．35）lg拷贝／mL（t=5．99，P〈0．01）。结论在体外细胞实验中，HBIG～PBCA—NP能抑制HBsAg、HBVDNA的分泌，且较原药HBIG作用有增强趋势。Objective To investigate the inhibitive activities of hepatitis B immunoglobulin （HBIG） poly （butyleynaoacrylate） nanoparticles （HBIG-PBCA-NP） to hepatitis t3 surface antigen （HBsAg） and hepatitis B virus （HBV） DNA secretions using HBV infected cell model in vitro. Methods HepG 2. 2. 15 cells were cultured with media containing HBIG-PBCA-NP or HBIG for several days, or cultured with HBIG-PBCA-NP and HBIG for 2 days and without HBIG-PBCA-NP and HBIG from day 3. The supernatants at different time points were collected for quantitative detection of HBsAg and HBV DNA. The comparisons between groups were done by variance analysis. Results Secretions of HBsAg and HBV DNA in supernatants of HepG2. 2. 15 cultured with 0.1- 10.0 IU/mL of HBIG-PBCA-NP and HBIG were inhibited significantly compared with control group. HBsAg titers and HBV DNA levels in supernatants of HBIG-PBCA-NP group and HBIG group cultured with media without HBIC-PBCA NP and HBIG kept decreasing at day 5 and 7, then rebounded at day 9 and 11. HBsAg titers in supernatants of 0. 1, 1.0, 5.0 IU/mL HBIG-PBCA-NP group were all significantly different from those in HBIG group at day 9 [（31. 31 ± 1. 98） μg/L vs （40.62±2.99） μg/L, （23. 79!1. 31） μg/L vs （36. 51μ±2. 12） μ/L, （19. 91±1. 74） μg/L vs （33.03±1.65） μg/L; F= 412. 24, P〈0. 01]. Conclusion HBIG-PBCA-NP can inhibit secretions of HBsAg and HBV DNA in vitro, which is more effective than HBIG.

关 键 词：肝炎 乙型 免疫球蛋白类 氰丙烯酸盐类 脂质体纳米结构 肝炎表面抗原 乙型 DNA 病毒 

分 类 号：R512.62[医药卫生—内科学] R977.6[医药卫生—临床医学]