双苄基异喹啉化合物Ys-96对阿霉素或长春新碱耐药株人癌细胞的逆转抗药性作用  

Reversal Effect of Ys96, a Bisbenzylisoquinoline, on Adriamycin or Vincristine Resistance in Human Cancer Cells in Vitro

作　　者：叶祖光[1] 孙爱续[1] 李春英[1] 薛宝云[1] 王智民[1] 仝燕[1] 冯浩[1] 梁爱华[1] 王金华[1] 杨庆[1] 

机构地区：[1]中国中医研究院中药研究所

出　　处：《中国中药杂志》1998年第7期427-428,共2页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金

摘　　要：目的：研究双苄基异喹啉化合物Ｙｓ９６对阿霉素或长春新碱耐药株人癌细胞抗药性的逆转作用。方法：在体外培养的药物敏感株ＭＣＦ７及其阿霉素耐药株ＭＣＦ７／Ａｄ以及药物敏感株ＫＢ及其长春新碱耐药株ＫＢＶ２００的体外试验系统中，采用ＭＴＴ法研究耐药株人癌细胞在Ｙｓ９６作用下对阿霉素或长春新碱敏感性（ＩＣ５０）的变化。结果：耐药株ＭＣＦ７／Ａｄ和ＫＢＶ２００在Ｙｓ９６（１００μｍｏｌ／Ｌ）的作用下，阿霉素和长春新碱对其ＩＣ５０值分别接近于二药单独用药时对敏感株ＭＣＦ７和ＫＢ的ＩＣ５０。结论：Ｙｓ９６基本上能完全逆转ＭＣＦ７／Ａｄ或ＫＢＶ２００对阿霉素或长春新碱的抗药性。Objective: Reversal effect of Ys96,a bisbenzylisoquinoline,on the resistance of human cancer cells to adriamycin or vincristine was studied in vitroMethod:In an in vitro culture system of human cancer cells MCF7 and its adriamycinresistant line MCF7/Ad or KB and its vincristineresistant line KBV200,the sensitivity(IC50)of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay.Result:IC50value of adriamycin or vincristine in combination with Ys96 at a concentration of 1.00μmol/L against MCF7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF7 or KBConclusion:The drug resistance of MCF7/Ad or KBv200 could be essentially reversed by Ys96 at a concentration of 100μmol/L

关 键 词：双苄基异喹啉 阿霉素 抗癌 多药抗药性 汉防己 

分 类 号：R285.5[医药卫生—中药学] R730.53[医药卫生—中医学]