电磁脉冲辐照后大鼠小脑、海马中一氧化氮合酶的变化  

Changes of Nitric Oxide Synthase in Hippocampus and Cerebellum of the Rat Following Exposure to Electromagnetic Pulse

作　　者：丁桂荣[1,2] 谢学军[1,2] 张李燕[1,2] 郑振兴[1,2] 周晏[1,2] 郭鹞[1,2] 

机构地区：[1]第四军医大学防原医学教研室 [2]西北核技术研究所

出　　处：《中华物理医学杂志》1998年第2期81-83,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的拟从观察电磁脉冲（ＥＭＰ）辐照后大鼠小脑、海马中一氧化氮合酶（ＮＯＳ）的表达情况，探讨ＥＭＰ所致大鼠学习、记忆能力改变的可能机制。方法采用ＳＰ免疫组织化学染色检测ＥＭＰ辐照后大鼠小脑、海马组织中ＮＯＳ阳性神经元的变化。结果ＥＭＰ辐照后１．５、２４ｈ海马中ＮＯＳ阳性神经元数目较对照组显著下降，且着色变浅，照后４８ｈＮＯＳ阳性神经元数目恢复至对照水平，但着色仍浅。小脑中ＮＯＳ阳性细胞在照后无明显变化。结论ＮＯＳ在ＥＭＰ所致大鼠学习。Objective In order to explore the role of nitric oxide in the obstruction of learning and memory of the rat caused by exposing to electromagnetic pulse(EMP),the distribution of nitric oxide synthase(NOS) expression was studied in hippocampus and cerebellum of the rat following exposure to EMP. Methods SP immunohistochemical staining was employed to detect the distribution of NOS expression. Results The number of NOS positive neurons and the intensity of positive staining in hippocampus were decreased at 1.5 and 24h after exposure to EMP. At 48h,the number of NOS positive neurons reversed to control level but the intensity of positive staining was still lower.The expression of NOS in cerebellum had no obvious changes.Conclusion Decrease of NOS expression in hippocampus relates to the obstruction of learning and memory of the rat after exposure to EMP.

关 键 词：电磁学 海马小脑 一氧化氮 代谢 

分 类 号：R318.03[医药卫生—生物医学工程] R312[医药卫生—基础医学]