人巨细胞病毒免疫刺激复合物制备技术方法的建立  

Establishment of the method for preparing HCMV ISCOMs

作　　者：刘志红[1] 黄文杰[1] 卜凤荣[1] 黄祥瑞 杨淑静[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1998年第2期141-143,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的：建立制备免疫刺激复合物（ＩＳＣＯＭｓ）的方法，ＩＳＣＯＭｓ是一种疫苗制备新技术，具有突出的免疫学价值。方法：本研究以人巨细胞病毒（ＨＣＭＶ）包膜蛋白和皂苷类佐剂ＱｕｉｌＡ作用，采用非离子去垢剂Ｍｅｇａ－１０及超速离心技术，进行了ＨＣＭＶＩＳＣＯＭｓ实验型疫苗的制备。结果：浓缩样品液经蔗糖梯度超速离心后，在离心管底部可见白色病毒带；纯化样品电镜观察具有典型的ＩＳＣＯＭｓ形态。结论：本研究制备样品在电镜下观察见抗原蛋白分布在ＩＳＣＯＭｓ颗粒表面，为直径３０ｎｍ左右的球状结构，与国外文献所报道形态相符。Objective: To establish the method for preparing immunostimulating complexes (ISCOMs) which is a new technology for preparing vaccine and has prominent value in immunology. Methods: In this study, the envelope proteins of HCMV were complexed with the glycoside Quil A by hydrophobic interaction. Mega 10 and sucrose gradient centrifugation were also used. Results: Sucrose density gradient centrifugation gave ISCOMs as a white sediment in the centrifuge tube. Electronmicroscopic examination showed that the preparation had the typical appearance and structure of ISCOMs. Conclusion: Under electronmicroscope the ISCOMs were spherical particles of 30 nm in diameter.

关 键 词：巨细胞病毒 免疫刺激复合物 超速离心法 疫苗 

分 类 号：R392-33[医药卫生—免疫学]