酸枣仁提取物对小鼠吗啡条件性位置偏爱的影响  

EFFECTS OF ACTIVE FRACTION OF SEMEN ZIZIPHI SHINOSAE ON MORPHINE INDUCED CONDITIONED PLACE PREFERENCE IN MICE

作　　者：余惠敏[1] 陈崇宏[1] 陈少雅[1] 

机构地区：[1]福建医科大学药学院药理学系,福州350004

出　　处：《中国药物依赖性杂志》2009年第3期180-184,共5页Chinese Journal of Drug Dependence

基　　金：福建省科技计划项目(2007F3039);福建省自然科学基金项目(C0410026)资助

摘　　要：目的:观察酸枣仁提取物对吗啡(MOR)诱导建立小鼠条件性位置偏爱(CPP)行为的影响。方法:连续给予吗啡(6mg·kg-1,sc)5d,引起小鼠产生显著的CPP效应,观察酸枣仁提取物对CPP效应的影响。结果:吗啡可诱导小鼠对伴药箱产生显著的位置偏爱;训练阶段于每次给予吗啡前30min给予酸枣仁醇提物800mg·kg-1可拮抗小鼠对吗啡产生的CPP效应(与吗啡对照组比较P<0.05),但酸枣仁醇提物200和400mg·kg-1不影响其效应(P>0.05);酸枣仁乙酸乙酯提取物13mg·kg-1、20mg·kg-1,正丁醇提取物160mg·kg-1可拮抗小鼠对吗啡产生的CPP效应(P<0.05),但酸枣仁石油醚提取物、乙醚提取物、水提物均不影响其效应(P>0.05)。结论:酸枣仁醇提物及其乙酸乙酯提取物、正丁醇提取物能在一定程度上抑制吗啡诱导小鼠建立的CPP行为。Objective：To investigate the effects of the active fraction of Semen Ziziphi Spinosae（SZS） on morphine - induced conditioned place preference （CPP） in mice. Methods ： Daily subcutaneous injection of morphine（6 mg·kg^-1 ） was administered to mice for 5 days to induce CPP in mice,and daily treatment with a series of extracts of SZS （ip） was given 30 min prior to the administration of morphine to observe the effect of SZS on morphine -induced CPP. Result： Morphine （6 mg·kg^-1, sc） induced mice to obtain a significant CPP and the CPP model was reliable and stable. By pretreating with alcoholic extract of SZS 800mg·kg^-1 （ but not 200mg·kg^-1 or 400mg·kg^-1 ）, ip, before morphine injection, the morphine - induced CPP was antagonized significantly（ P 〈 0. 05）. Then by pretreating with acetoacetate（ 13 mg·kg^-1,20 mg·kg^-1 ）, and n - butanol （ 160mg·kg^-1） extract of SZS, the CPP was also inhibited （ P 〈 0. 05 ）, but the CPP was not affected by petroleum benzin, ethereal and aqueous extract of SZS. Conclusion ： The effects of the active fraction of SZS can suppress the acquisition of morphine induced CPP in mice.

关 键 词：酸枣仁 吗啡 条件性位置偏爱 

分 类 号：R978.7[医药卫生—药品] R595.5[医药卫生—药学]