RP-HPLC法同时测定维血宁颗粒中芍药苷、虎杖苷、麦角甾苷和白藜芦醇的含量  

RP-HPLC simultaneous determination of paeoniflorin,verbascoside,polydatin and resveratrol in Weixuening granules

作　　者：朱鸿敏[1] 金艺[1] 李晶[1] 鲁樱[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2009年第6期905-908,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立RP-HPLC法同时测定维血宁颗粒中芍药苷、虎杖苷、麦角甾苷和白藜芦醇4种成分的含量。方法:采用Di-amonsil C18(200mm×4.6mm,5μm)色谱柱;以乙腈-0.1%磷酸溶液为流动相梯度洗脱;流速1.0mL·min-1;柱温35℃;检测波长303nm。结果:芍药苷、虎杖苷、麦角甾苷和白藜芦醇4种成分浓度分别在22.3～223,61.1～611,6.38～63.8,4.88～48.8μg·mL-1范围内与峰面积呈良好线性关系(r>0.9995);方法平均回收率(n=9)分别为99.5%,98.6%,101.5%,100.2%。结论:本法简便、准确,重复性好,可用于维血宁颗粒的质量控制。Abstract side and C18 （200 Objective： To establish a method for the simultaneous determination of paeoniflorin, polydatin, verbascoresveratrol in Weixuening granules by RP - HPLC. Methods： The analysis was performed on a Diamonsil mm ×4. 6 mm,5 μm） column. The mobile phase was composed of acetonitrile and 0. 1% phosphoric acid solution in gradient elution mode with at a flow rate of 1.0 mL · min^-1. The column temperature was 35 ℃ and the UV detection wavelength was 303 nm. Results：The linear ranges of paeoniflorin, polydatin, verbascoside and resveratrol were 22. 3 - 223,61.1 - 611,6. 38 - 63.8,4. 88 - 48.8μg · mL^-1（ r 〉 0. 9995 ） , respectively. The average recoveries（ n = 9 ） of paeoniflorin, polydatin, verbascoside and resveratrol were 99.5%, 98.6%, 101.5% and 100. 2%, respectively. Conclusion ： This method is simple accurate and reproduceable which can be used for the quality control of Weixuening granules.

关 键 词：维血宁颗粒 芍药苷 虎杖苷 麦角甾苷 白藜芦醇 RP-HPLC 

分 类 号：R917[医药卫生—药物分析学]