RP-HPLC法测定千金子中3种千金二萜醇酯的含量  

RP-HPLC determination of three diterpenoids in seeds of Euphorbia lathyris L.

作　　者：孙洪峰[1] 刘桂英[1] 孙婷[1] 魏巍[1] 周洪玉[1] 李绪文[1] 马双成[2] 金永日[1] 

机构地区：[1]吉林大学化学学院,长春130021 [2]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2009年第6期909-911,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:对千金子中千金子素L1(化合物Ⅰ)、千金子素L2(化合物Ⅱ)和千金子素L3(化合物Ⅲ)3种千金二萜醇酯进行含量测定。方法:采用反相高效液相色谱法,使用Prevail-C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(75∶25),流速1.0mL·min-1,检测波长280nm,柱温25℃。结果:化合物Ⅰ、Ⅱ、Ⅲ进样量均在0.1～4.0μg范围内有良好的线性关系;相关系数r分别为0.9998,0.9999,0.9996;平均回收率(n=6)分别为97.9%(RSD为1.5%),100.9%(RSD为1.5%),99.6%(RSD为1.2%)。结论:本方法能够准确测定千金子中化合物Ⅰ、Ⅱ、Ⅲ3种千金二萜醇酯的含量。Objective：To determine three diterpenoids, euphorbia factor L1（compound Ⅰ ）, euphorbia factor L2 （ compound Ⅱ ） and euphorbia factor L3 （ compound Ⅲ ） in seeds of Euphorbia lathyris L.. Methods： Separation was carried out on Prevail- C18 column（250 mm ×4. 6 mm,5 μm）. The mobile phase was methanol- water（75： 25 ）. The flow rate was 1.0 mL · min^-1 at 25 ℃, and the detection wavelength was set at 280 nm. Results：The linearity was in the range of 0.1 - 4.0 g for compounds Ⅰ, Ⅱ, Ⅲ and the correlation coefficients were 0.9998,0. 9999 and 0. 9996 ,respectively. The average recoveries （ n = 6） for compounds Ⅰ, Ⅱ, Ⅲ were 97.9% （ RSD =1.5 % ）, 100. 9% （RSD = 1.5 % ） ,99.6% （RSD = 1.2% ）, respectively. Conclusion：This method is accurate, reliable and sensitive for determination of three diterpenoid in seeds of Euphorbia lathyris.

关 键 词：千金子 千金二萜醇酯 千金子素 反相高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]