HPLC法测定白花前胡甲素在大鼠肝微粒体中的含量  

HPLC determination of praeruptrum A(Pd-Ia) in rat hepatic microsomal fraction

作　　者：邹军[1] 胡新颖[2] 朱铉[1] 普布赤列 高学敏[1] 

机构地区：[1]厦门大学医学院,厦门361005 [2]延边大学药学院,延吉133000 [3]西藏自制区林芝食品药品监督管理局,林芝860000

出　　处：《药物分析杂志》2009年第6期923-925,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：福建省自然科学基金(2008J0015);福建省新世纪优秀人才支持计划项目

摘　　要：目的:建立一种快速准确检测大鼠肝微粒体中白花前胡甲素(Pd-Ia)含量的方法。方法:以华蟾毒精为内标物,采用Hypersil-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水(75∶25)为流动相,流速1mL·min-1,检测波长321nm,柱温25℃,进样量20μL。结果:Pd-Ia浓度在1～100μg·mL-1范围内与峰面积呈现良好的线性关系(r=0.9989);日内精密度RSD≤2.9%;日间精密度RSD≤8.5%;平均加样回收率(n=9)为98.4%。结论:本文建立的方法操作简单,可用于Pd-Ia含量的测定。Objective：To establish a method of determination of praeruptrum A（ Pd -Ia）in rat hepatic microsomes fraction by HPLC. Method： Hypersil -C18（250 mm × 4. 6 ram,5 μm） column was adopted at a temperature of 25 ℃. An HPLC method was established using cinobufagin as internal standard. The mobile phase consisted of methanol - water （75：25 ） at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 321 nm,the injection volume was 20 μL. Results：The calibration curve was shown to be a linear over the range of 1 - 100 μg ·mL^-1 （ r = 0. 9989 ）. The mean recovery（ n = 9 ） was 98.4%, The RSDs of intra - day and inter - day were less than 2. 9% and 8.5%, respectively. Conclusion ：The method is specific, simple and has been successfully applied to the determination of Pd - Ia.

关 键 词：高效液相色谱法 白花前胡甲素(Pd-Ia) 华蟾毒精 大鼠 肝微粒体 

分 类 号：R917[医药卫生—药物分析学]