RP-HPLC法测定香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛的含量  

RP-HPLC determination of isovanillin,periplocin and 4-methoxy salicylaldehyde in root bark of Cortex Periplocae

作　　者：佟玲[1] 谭晓杰[1] 林景瑞[1] 陈晓辉[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2009年第6期961-963,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛含量的方法。方法:采用RP-HPLC法,Hypersil ODS2色谱柱(250mm×4.6mm,5μm,大连依利特);流动相为乙腈(A)-0.02%磷酸水溶液(B)系统,梯度洗脱[0～10min,85%B;10～40min,85%B→70%B;40～60min,70%B→50%B];流速:1.0mL·min-1;检测波长为230nm。结果:异香草醛、杠柳毒苷和4-甲氧基水杨醛进样浓度分别在1.65～13.7,5.91～49.2,4.97～99.4μg·mL-1范围内与峰面积呈良好的线性关系(r分别为0.9996,0.9994,0.9996);方法回收率(n=9)分别为101.7%,99.5%,99.9%。结论:该方法简便、准确,重复性好,适用于香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛的定量分析。Objective ：To establish a method for simultaneous determination of isovanillin, periplocin and 4 - methoxy salieylaldehyde in Cortex Periplocae. Methods：The analysis was performed on a Hypersil ODS2 column （250 mm 4. 6 mm,5 μm）. The mobile phase consisted of acetonitrile （A） and 0. 02% aqueous phosphoric acid （B）,with gradient program adopted as follows ： 0 - 10 min, 85% B ; 10 - 40 min, 85% B--→70% B ; 40 - 60 min, 70%→50% B. The flow rate was maintained at 1.0 mL · min^ -1 and the detection wavelength was set at 230 nm. Results：The linear response ranges were 1.65 - 13.7 μg · mL^-1 for isovanillin （r = 0. 9996 ） , 5.91 -49. 2 μg · mL^-1 for periplocin （ r = 0. 9994 ） and 4. 97 - 99.4 μg · mL^-1 for 4 - methoxy salicylaldehyde （ r = 0. 9996）. The recoveries （n = 9） of these compounds were 101.7% ,99. 5 % and 99. 9%, respectively. Conclusion：The method is simple, accurate and reproducible for quality control of Cortex Periplocae.

关 键 词：香加皮 反相高效液相色谱法 异香草醛 杠柳毒苷 4-甲氧基水杨醛 

分 类 号：R917[医药卫生—药物分析学]