反相离子对高效液相色谱法测定更年舒片中维生素B_6的含量  

Ion-pair RP-HPLC determination of vitamin B_6 in Gengnianshu tablets

作　　者：王英锋[1] 梁建贞[1] 张建博[1] 

机构地区：[1]首都师范大学分析测试中心,北京100037

出　　处：《药物分析杂志》2009年第6期1019-1021,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用反相离子对高效液相色谱法测定更年舒片中维生素B6的含量。方法:色谱柱:YM Cpacked ODS-A(250mm×4.6mm,5μm);流动相:甲醇-0.1%己烷磺酸钠(用冰醋酸调pH=3.1)(4∶96);流速:1.0mL·min-1;检测波长:291nm;柱温:25℃。结果:维生素B6与其他成分分离良好。维生素B6在0.15～0.76μg范围内呈良好的线性关系(r=0.9998),平均回收率(n=9)101.3%。结论:方法简便、准确、重现性好。Objective：To develop an RP - HPLC method for the determination of vitamin B6 in Gengnianshu tablets. Methods：A YMC packed ODS-A（250 mm×4. 6 mm,5 μm）column was used,with mobile phase consisted of methanol -0. 1% sodium hexanesulfonate （4： 96, adjusted to pH 3. 1 with glacial acetic acid）. The flow rate was 1.0 mL · min^-1. The detecting wavelength was 291 nm. The column temperature was 25 ℃. Results： Vitamin B6 was well separated. The calibration curve was linear over the concentration range of 0. 15 -0. 76μg（ r = 0. 9998）. The average recovery of vitamin B6 （ n = 9 ） was 101.3%. Conclusion： The method is simple, accurate and reproducible.

关 键 词：更年舒片 维生素B6 反相离子对高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]