HPLC法测定肿节风中异秦皮啶含量  

Determination of Isofraxidin in Sarcandra glabra(Thunb.)Nakai by HPLC

作　　者：张萍[1] 李军 马双成[1] 王钢力[1] 林瑞超[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]安徽省亳州药品检验所

出　　处：《中国药事》2009年第6期538-540,共3页Chinese Pharmaceutical Affairs

摘　　要：目的测定肿节风药材中异秦皮啶含量。方法样品经甲醇加热回流提取,采用HPLC法测定。色谱柱为Alltima C18柱;流动相：乙腈-0.1%磷酸溶液（20∶80）;流速：1.0mL.min-1;检测波长：344nm。结果异秦皮啶在0.021~0.128μg范围内具有良好的线性关系（r=0.9994）;平均加样回收率（n=5）为99.4%,RSD=2.62%。结论本方法操作简便、快捷,灵敏度高,可用于肿节风中异秦皮啶的含量测定。Objective To determine isofraxidin in Sarcandra glabra （Thunb.） Nakai. Methods The contents of isofraxidin in Sarcandra glabra （Thunb.） Nakai were assayed by HPLC. The column used was Alltima C18 column, the mobile phase was acetonitrile-0. 1% phosphoric acid （20 ： 80） at a flow rate of 1.0mL · min^-1, the detection wavelength was set at 344nm. Results The linear range was 0. 021 0. 128μg （r=0. 9994）. The average recovery （n= 5） of isofraxidin was 99.4%, RSD was 2.62%. Conclusions The method is effective and accurate. It can be applied to determination of isofraxidin in Sarcandra glabra （Thunb.） Nakai.

关 键 词：肿节风 异秦皮啶 HPLC 

分 类 号：R284.2[医药卫生—中药学] R927.2[医药卫生—中医学]