HPLC法测定联磺甲氧苄啶片的含量  

Determination of Sulfamethoxazole,Sulfadiazine and Trimethoprim Tablets by HPLC

作　　者：魏京京[1,2] 张启明[1] 金少鸿[1] 

机构地区：[1]中国药品生物制品检定所 [2]中国医学科学院协和医科大学研究生院

出　　处：《中国药事》2009年第6期565-567,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立了同时测定联磺甲氧苄啶片中3个组分含量的高效液相色谱法。方法色谱柱为DikmaDiamonsil C18;流动相为甲醇-乙酸铵溶液（33∶67）,检测波长为210nm;流速为1mL.min-1。结果磺胺甲噁唑、磺胺嘧啶和甲氧苄啶的线性范围分别为50~150μg.mL-1（r=1.000）、50~150μg.mL-1（r=0.9999）、10~30μg.mL-1（r=0.9998）;平均回收率分别为101.6%（RSD=1.20%,n=9）、98.4%（RSD=0.95%,n=9）、98.7%（RSD=1.03%,n=9）。结论本法简单、快速、准确,可用于联磺甲氧苄啶片的定量分析。Objective A HPLC method was established to determination of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets. Methods Stationary phase was Dikma Diamonsil C18, mobile phase was methanol-ammonium acetate solution （33 ： 67）, the flow rate was 1mL · min^-1. Results There was a good linear relationship when the content of sulfamethoxazole, sulfadiazine and trimethoprim were within the range of 50-150μg · mL^-1（r= 1.000）, 50-150μg · mL^-1 （r=0. 9999） and 10-30μg · mL^-1 （r=0. 9998） respectively. The average recovery were 101.6%（RSD=1. 20%,n=9）, 98.4%（RSD=0.95%,n= 9） and 98. 7%（RSD=1.03% ,n=9） respectively. Conclusions This method is simple, rapid and accurate. It can be used for the quality control of Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets.

关 键 词：联磺甲氧苄啶片 磺胺甲噁唑 磺胺嘧啶 甲氧苄啶 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]