改良QuEChERS-气相色谱法快速检测血中有机磷农药的方法研究  

Detection of organophosphorus pesticides in blood by QuEChERS-GC

作　　者：洪萍[1] 李颖[1] 李峰[1] 徐陆妹[1] 应杏秋[1] 韩见龙[2] 

机构地区：[1]杭州市萧山区疾病预防控制中心,杭州311201 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2009年第6期1296-1298,共3页Chinese Journal of Health Laboratory Technology

基　　金：萧山区科技计划(2008)

摘　　要：目的：建立一种同时测定中毒者血样中4种有机磷农药的QuEChERS-气相色谱快速检测方法。方法：血样先用甲酸沉淀杂质后,用乙酸乙酯提取,经活性炭、PSA、PEP混合型固相分散净化后,取上清液测定。结果：4种有机磷农药浓度在0.035～3.50μg/m l范围,其线性相关系数在0.9981～0.9999之间,样品加标回收率为73.1%～119.6%,RSD为3.2%～12.6%,最低检出限在0.01～0.02 mg/L之间。结论：该方法简便、快速、回收率高,可满足血样中有机磷农药的快速检测要求。Objective ： To develop a new method to detect four kinds of organophosphorus pesticides in blood by QuEChERS - GC. Methods：The impurities in blood samples were removed by formic acid firstly, then the blood were extracted with ethyl acetate and cleaned up by dispersive - SPE with activated carbon, PSA and PEP and the supernatant were determined. Results： The linear range was 0. 035 - 3.50 μg/ml （ r = 0. 9981 - 0. 9999）. The recovery range was 73.1% - 119.6%. RSD was 3.2% 12. 6% , and the lowest detecting limits was 0. 01 -0. 02 mg/L. Conclusion：The method can be used to analyze organopbosphorus pesticides in blood with convenience, rapidity, and high recovery rate.

关 键 词：血样 有机磷农药 QUECHERS 快速检测 

分 类 号：O657.71[理学—分析化学]