河南艾滋病病毒基因型耐药检测研究  

Analysis of genotype and drug resistance in HIV-1 in Henan Province

作　　者：朱新朋[1] 刘宏伟[1] 田随安[1] 袁源[1] 刘春华[1] 崔为国[1] 朱谦[1] 王哲[1] 

机构地区：[1]河南省疾病预防控制中心性病艾滋病防治研究所,郑州450016

出　　处：《中国卫生检验杂志》2009年第6期1324-1326,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:检测研究河南省艾滋病病毒基因型耐药变异发生的情况。方法:采集262例HIV/AIDS患者抗凝全血,用扩增/测序(In-House)的方法扩增HIV-I基因蛋白酶及逆转录酶区基因后进行基因序列测定,提交斯坦福耐药数据库分析基因耐药性突变情况。结果:未接受抗病毒治疗的HIV/AIDS患者34.62%发生耐药突变,蛋白酶区耐药突变率为28.85%,逆转录酶区耐药突变率为17.31%;接受抗病毒治疗的HIV/AIDS患者43.81%发生基因耐药突变,蛋白酶区基因耐药突变率为21.43%,逆转录酶区突变率为38.10%。结论:河南省确山县HIV/AIDS患者部分出现了基因型耐药,应加强HIV/AIDS患者管理及耐药检测体系,减少耐药毒株及患者的产生。Objective：To investigate genotype and drug resistance in HIV/AIDS infectors in Henan Province. Methods：262 samples were separated from the anticoagulatory whole blood, and in - house technique was used to detected the genotype drug resistance. The sequences were submitted to stanford drug resistance database to analyz. Results： The mutation rate of drug resistance was 34. 62% （ 18/52）of HIV/AIDS infectors,the minor mutation rate of resistance to protease was 28.85% （ 15/52）, the mutation rate of resistance to reverse transeriptase was 17. 31% （9/52）. The mutation rate of drug resistance was 43.81% （ 92/ 210） of HIV/AIDS patients treated with antiretroviral drugs, the minor mutation rate of resistance to protease was 21.43% （45/ 210 ） , the mutation rate of resistance to reverse transcriptase was 38. 10% （80/210）. Conclusion： Some of HIV/AIDS patients in Henan province were genotype drug resistance to the currently available antiviral medicine. The system of antiviral management and sruvillianee should to be adjusted to decrease the transmission of drug resistant virus.

关 键 词：艾滋病病毒 耐药性 蛋白酶 逆转录酶 

分 类 号：R512.91[医药卫生—内科学]