体检用真空采血管带菌情况检测  

Herm-carrying condition of vacuum blood tubes used in physical examination

作　　者：付秀萍[1] 张守印[1] 亓晓[2] 张景山[1] 贺金荣[1] 海荣[1] 俞东征[1] 

机构地区：[1]传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所,北京102206 [2]中国疾病预防控制中心流行病学办公室,北京100050

出　　处：《中国卫生检验杂志》2009年第6期1378-1379,共2页Chinese Journal of Health Laboratory Technology

基　　金：国家科技攻关计划课题(2003BA712A02)

摘　　要：目的:检测体检用真空采血管中是否带菌以及其带菌情况。方法:对7个批号真空采血管进行细菌培养后,提取染色体,利用16 s rRNA细菌通用引物进行PCR扩增和序列分析。结果:检测的采血管中有5个批号都检出带菌,培养阳性率平均为32.3%,所带细菌大多为对营养条件要求比较低的细菌,如副球菌、地衣芽孢杆菌、葡萄球菌、假单胞菌等。结论:体检用真空采血管普遍带菌,不能用于与细菌有关的各种检测,需要使用真空采血管时必须使用带有无菌标志的专用采血管。Objective ：To assess the germ -carrying condition of the vacuum blood tubes used in sample collection. Methods： We performed germ - culture with vacuum blood tubes of 7 different batch number and abstract that of genome. The 16S rRNA gene sequence was amplified with universal primers and the amplified products were then sequenced. ResultS：Among the 7 batch number of vacuum tubes examined, bacteria were detected from vacuum blood tubes of 5 batch numbers, with an average positive rate of 32. 3%, most of which were low -trophic bacteria. Conclusion：These data suggest that vacuum blood tubes used in physical examination is germy in general ,so tests relating to bacteria must use special blood tubes marked with aseptic.

关 键 词：采血管 细菌 聚合酶链反应 

分 类 号：R378[医药卫生—病原生物学]