RT—套式PCR法检测病毒性肝炎患者血清中HGV-RNA  

DETECTION OF HGVRNA IN SERA Of PATIENTS WITH VIRAL HEPATITIS BY RT-NESTED PCR ASSAY

作　　者：刘泽明[1] 陈慧[1] 张建军[1] 史小玲[1] 彭建一[1] 王明勇[1] 钟森[1] 

机构地区：[1]泸州医学院附属医院传染科,646000

出　　处：《泸州医学院学报》1998年第1期14-16,共3页Journal of Luzhou Medical College

摘　　要：目的：为了解四川泸州地区庚型肝炎病毒（HGV）流行情况，方法：根据发表的HGV序列非编码区（NCR）设计两对引物，用RT-套式PCR方法，结果：对102例各型肝炎病人血清进行检测，发现39例HGV-RNA阳性，阳性率为38.23％。其中3例（7.69％）HGV-RNA阳性者为非A-E型肝炎，33例（84.61％）为HBV与HGV共同感染，另外3例（7.69％）为HCV和HGV共同感染。结论：提示泸州地区是HGV高流行区。PCR所得产物进行序列测序，结果表明，“泸州株”HGV非编码区与“中国株”同源性达89.4％，与“重庆株”同源性达94.3％，提示“泸州株”和“重庆株”可能为相同亚型。Objective: Epidemiological survey of HGV in Luzhou area. Methods: A set of primers designed according to the NCR of HGV-RNA was subjected to RT-ncsted PCR assay to detect the HGV-RNA in sera of 1O2 patients with various viral hepatitis. Results: 39 serum samples were HGV-RNA positive; the positive proportion was up to 38. 23 perccnt. Of these 39 HGV-RNA positlvc patients,HNA～E were 3 (7. 69 % ), HB 33 (84. 61 % ), the others (3 cases, 7. 69 % ) were coinfected with HCV and HGV. Conclusion: The sequence analysis of PCR product of Luzhou HGV isolate showed an 89. 4% nucleotide identity ovcr the corresponding region of Chinese HGV, and a 94. 3% nucleotide identity over Chongqing isolate,indicating that Lzhou HGV isolate and Chongqing isolate belong to the same HGV subtype. The spread of HGV in Luzhou area is as high as that in Chongqing.

关 键 词：病毒性肝炎 庚型肝炎病毒 RNA PCR 

分 类 号：R512.604[医药卫生—内科学]