Concentration of α-Linoleic Acid of Perilla Oil by Gradient Cooling Urea Inclusion  

作　　者：GU Hai-bo MA Xue-yi WU Jing-bo ZHANG Qi YUAN Wen-bing CHEN Yi-ping 

机构地区：[1]Key Study Center of the National Ministry of Education for Tropical Resources Utilization/Hainan Provincial Key Labortary of Fine Chemistry, Haikou 570228, P.R. China [2]State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P.R.China [3]Department of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R.China

出　　处：《Agricultural Sciences in China》2009年第6期685-690,共6页中国农业科学（英文版）

基　　金：the National Natural Science Foundation of China (20871061);the National 973 Program of China (2008CB617512) for the financial support

摘　　要：In this study, production of ct-linoleic acid concentrated from crude perilla oil by gradient cooling urea inclusion was optimized. The fatty acid composition was determined after ethyl esterification by gas chromatography （GC）. In this process, orthogonal experiment was carried out. Under optimum conditions, the maximum amount of α-linoleic acid （91.5%） was obtained at a urea to fatty acid ratio of 3, a solvent to fatty acids ratio of 7, a reaction temperature of 348 K and a crystallization time of 690 min. A simple method of gradient cooling urea inclusion was used to purify α-linolenic acid by using urea to form inclusion complexes with the saturated and the less unsaturated fatty acids, which enhanced the purity of α-linoleic acid ethyl ester by above 90%.In this study, production of ct-linoleic acid concentrated from crude perilla oil by gradient cooling urea inclusion was optimized. The fatty acid composition was determined after ethyl esterification by gas chromatography （GC）. In this process, orthogonal experiment was carried out. Under optimum conditions, the maximum amount of α-linoleic acid （91.5%） was obtained at a urea to fatty acid ratio of 3, a solvent to fatty acids ratio of 7, a reaction temperature of 348 K and a crystallization time of 690 min. A simple method of gradient cooling urea inclusion was used to purify α-linolenic acid by using urea to form inclusion complexes with the saturated and the less unsaturated fatty acids, which enhanced the purity of α-linoleic acid ethyl ester by above 90%.

关 键 词：gradient cooling α-linoleic acid urea inclusion fatty acid perilla oil 

分 类 号：TQ645.6[化学工程—精细化工] TQ463.2