经皮穴位电刺激对肢体缺血再灌注损伤氧自由基系统的影响  

Effect of Percutaneous Electrical Stimulation of Acupuncture Points on Blood Oxygen Free Radical Induce by Ischemia-reperfusion Injury in Limbs

作　　者：黄金平 幸志强 

机构地区：[1]广东省佛山市南海里水医院麻醉科,广东佛山528244 [2]暨南大学第二临床学院深圳市人民医院麻醉科,深圳518020

出　　处：《中国现代医生》2009年第18期4-5,共2页China Modern Doctor

摘　　要：目的研究经皮穴位电刺激对肢体缺血再灌注损伤氧自由基系统的影响。方法30例择期行单侧下肢手术患者随机分为A、B两组,每组15例。两组均采用腰麻-硬膜外联合阻滞麻醉。B组于麻醉前以韩氏刺激仪刺激双侧内关、外关至手术结束。分别于麻醉前(T0)、放止血带后10(T1)、30(T2)、60(T3)min四个时段抽取肘静脉血测定丙二醛(MDA)、谷胱甘肽(GSH)及超氧化物歧化酶(SOD)的浓度。结果两组患者性别、年龄、体重、止血带时间等差异无统计学意义,麻醉前两组MDA、GSH、SOD无明显差异。与T0相比,两组T1、T2、T3时MDA均显著增高(P<0.01),GSH、SOD则显著降低(P<0.01)。与A组比较,B组MDA在T1、T2、T3各时点显著降低(P<0.01),GSH、SOD均显著增高(P<0.01)。结论经皮穴位电刺激对肢体缺血再灌注损伤起到一定的保护作用。Objective To study the effect of percutaneous electrical stimulation of acupuncture points on blood oxygen free radical induce by ischemia-reperfusion injury in limbs. Methods 30 patients who received the unilateral lower limb surgery were randomly allocated to A and B groups equality. All the patients were received spinal-epidural anesthesia. Before the anesthesia,B groups patients were stimulated bilateral at NeiGuan and WaiGuan acupuncture points by HAN stimulate instruction until the end of the operation. The concentration of malondialdehyde（MDA）, glutathione（GSH） and superoxide dismutase（SOD） were measured by venous blood draw from their elbow in four different periods-before the anesthesia（To）, 10 minutes after the tourniquet released（T,）, 30 minutes after the tourniquet released（T2） and 60 minutes after the tourniquet released（T3） respectively. Results Gender, age, weight and the time of using tourniquet shows no different between-group differents. There is also no significant different between two groups before the anesthesia in MDA, GSH and SOD. The two groups all shows obviously increase of MDA（P〈0.01） and significant decrease of GSH and SOD （P〈0.01） in T1, T2 and T3 in contrast to in To. compare with the A group, B group shows a significant decrease of MDA （P〈0.01） and a significant increase in GSH and SOD （P〈0.01） in T1,T2,T3. Conclusion The percutaneous electrical stimulation of acupuncture points can protect the limb by ischemia-reperfusion injury.

关 键 词：经皮穴位电刺激 缺血再灌注损伤 氧自由基 丙二醛 谷胱甘肽 超氧化物歧化酶 

分 类 号：R245.9[医药卫生—针灸推拿学]