原花青素预处理对体外人源细胞辐射损伤的防护作用  

Protective effects of proanthocyanidin on radiation-induced injury of human-derived cells

作　　者：高福[1] 李百龙[1] 孙顶[1] 黄越承[1] 倪谨[1] 崔建国[1] 赵芳[1] 蔡建明[1] 

机构地区：[1]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《第二军医大学学报》2009年第6期641-644,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30470415;30800284)~~

摘　　要：目的:探讨原花青素(PC)预处理对体外人淋巴母细胞AHH-1和肠腺上皮细胞HIEC电离辐射损伤的防护效果及可能的作用机制。方法:采用CCK-8法检测原花青素预处理对γ射线照射后AHH-1、HIEC细胞存活率的影响,Annexin-Ⅴ/PI染色和流式细胞术分析细胞凋亡及细胞周期的变化,蛋白质印迹法检测细胞Bcl-2蛋白表达变化。结果:PC预处理的细胞辐照后存活率明显升高(P<0.01),4Gy照射用药组AHH-1细胞凋亡率(11.78%)显著低于单纯照射组(26.38%,P<0.01),8Gy照射用药组HIEC细胞凋亡率(5.32%)亦显著低于单纯照射组(12.45%,P<0.01)。PC预处理组AHH-1、HIEC细胞受照射后其Bcl-2蛋白表达下降受到抑制。结论:PC预处理对细胞的辐射损伤具有较好的防护效果,可能与其促进受损细胞Bcl-2蛋白表达有关。Objective：To investigate the protective effects of proanthocyanidin on radiation-induced injury of AHH-1 and HIEC cells,and to explore the possible molecular mechanism. Methods： CCK-8 assay was applied to examine the survival of AHH-1,HIEC cells after 7-ray irradiation with or without proanthocyanidin pretreatment. Annexin-V/PI staining and flow cytometry analysis were used to evaluate the apoptosis and cell cycle of AHH 1 and HIEC cells. The expression of Bcl-2 protein was analyzed by Western blotting assay. Results： Pretreatment with proanthocyanidin significantly increased the cell survival rate after radiation（P〈0.01）. The apoptosis rates of cells in the proanthocyanidin＋ radiation group were （11.78 % at 4 Gy[AHH- 1] and 5.32% at 8 Gy[HIEC],respectively） greatly lower than those of the single radiation group （26.38% at 4 Gy[AHH-1] and 12.45% at 8 Gy[HIEC],respectively,P〈0.01）. Moreover,the inhibition of Bcl-2 protein expression in AHH-1 ,HIEC cells was attenuated in the proanthocyanidin ＋ radiation group. Conclusion： Proanthocyanidin has satisfactory protective effect on radiation-induced cellular injury; the mechanism may be related to the attenuation of Bcl-2 protein inhibition.

关 键 词：原花青素类 辐射损伤 淋巴母细胞 肠腺上皮细胞 BCL-2蛋白 

分 类 号：R818.8[医药卫生—放射医学]