二巯基丁二酸与维生素C、蛋氨酸合用对染铅小鼠的影响  

Effects of DMSA Combined with Vitamin C and L-methioine on Concentration of Lead in Blood and Morphological Changes of Liver Cells in Lead-intoxicated Mice

作　　者：朱加银[1] 赵惠玲[1] 管敏强[1] 王一龙[1] 陈通克[1] 任晓丽[1] 楼文文[2] 陈锡文[1] 

机构地区：[1]温州医学院实验动物中心,浙江温州325035 [2]温州医学院附属第二医院检验科,浙江温州325000

出　　处：《实验动物与比较医学》2009年第3期177-180,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的探讨二巯基丁二酸（DMSA）与维生素C、蛋氨酸联合应用对染铅ICR小鼠血铅及肝脏的影响。方法实验组小鼠饮用0.1％的醋酸铅溶液6周后，随机分为模型对照组、低剂量DMSA组、高剂量DMSA组及低剂量DMSA与维生素C、蛋氨酸联合应用组（联合组）。给药4周后，测小鼠的血铅。并取肝脏组织进行苏木素-伊红（HE）染色，观察肝脏的形态学改变。结果低剂量DMSA组、高剂量DMSA组与联合组小鼠血铅浓度和模型对照组差异有显著性（P〈0.01）；正常对照组血铅浓度和模型对照组差异有显著性（P〈0.01）。肝病理学表现为肝细胞可见变性水肿，其中以联合组水肿程度最轻。结论DMSA与维生素C、蛋氨酸联合应用对小鼠体内铅的排泄具有更好的效果。Objective To investigate the combination effects of dimercaptosuccinic acid（DMSA）, vitamin C and L-methioine on the ICR mice induced by lead. Methods The mice of the experimental group were exposed to 0.1% lead acetate in drinking water for 6 weeks, which were randomly divided into model control group ,low dosage group of DMSA, high dosage group of DMSA and group of low dosage of DMSA combined with vitamin C and L-methioine. After 4 weeks of treatment ,the concentration of lead in blood was detected, and the morphological changes of liver cells was observed by Hema-toxylin and Eosin Staining. Results The difference of the concentration of lead in blood of model control group was more higher than those of low dosage group of DMSA ,high dosage group of DMSA or group low dosage of DMSA combined with vitamin C and L-methioine （P〈0.01）. The difference of the concentration of lead in blood between normal group and model control group was very significant （P〈0.01）. The liver cells appeared hydropic degeneration and the effect of combination group was significantly superior to other groups. Conclusion The effect of lead excretion for treating of DMSA combined with vitamin C and L-methioine more better than others.

关 键 词：铅 二巯基丁二酸 维生素C 蛋氨酸 

分 类 号：Q95-33[生物学—动物学]