催乳素受体基因多态性对西藏小型猪繁殖性能的影响  

Relationship between Polymorphisms of PRLR Gene and Reproductive Performance in Tibet Mini-Pigs

作　　者：曾昭智[1] 江涛[1] 张锦红[1] 顾为望[2] 

机构地区：[1]广东药学院实验动物中心,广东广州510006 [2]南方医科大学实验动物中心,广东广州510515

出　　处：《实验动物与比较医学》2009年第3期181-184,共4页Laboratory Animal and Comparative Medicine

基　　金：广东省科技计划项目（2005860302007）

摘　　要：目的了解催乳素受体（PRLR）基因AIuI位点在西藏小型猪中的多态性分布情况；研究其在西藏小型猪上的遗传效应，为生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法收集44头西藏小型猪母猪窝产仔数及离乳仔数数据，测定所产仔猪的初生重和离乳重，采用PCR-RFLP方法测定PRLR基因AIuI位点多态性，并与上述生长繁殖性能指标进行相关性分析。结果西藏小型猪PRLR侯选基因AIuI位点表现出多态性，分为AA、AB、BB，频率分别为0.273、0.500、0.227。经产母猪的仔猪初生重在不同基因型间存在显著性差异，而初产和经产母猪的其他生长繁殖指标在不同基因型间均未达到显著性水平。结论本研究中PRLR基因AIuI位点多态性AA、AB、BB不同基因型之间窝产仔数和离乳仔数差异不显著。Objective To obtain the information of AIu I polymorphism distribution of PRLR gene and to study its genetic effect on growth and reproductive performance in Tibet mini-pigs. Methods The litter size and numbers weaning, data of birth weight and weaning weight of piglet from 44 Tibet mini-pig sows were recorded. The AIu I polymorphism of PRLR gene was detected by PCR-RFLP method, and the relationship between the gene polymorphism and the growth and reproductive performance was analyzed by SPSS10.0. Results 3 genotypes ofAA, AB, BB were detected in 44 Tibet mini-pigs, the frequency of genotypes was 0.273, 0.500, 0.227 respectively. Except there was a significant difference in birth weight of latter parity between different genotypes, the other index of growth and reproductive performance between different genotypes had no significance difference. Conclutions There was no significant difference on litter size and numbers of weaning between different genotypes of Tibet mini-pigs.

关 键 词：PRLR基因 多态性 西藏小型猪 生长繁殖性能 

分 类 号：Q95-33[生物学—动物学]