皮质酮对大鼠早期再生肝抗酶mRNA及蛋白表达的影响  

Effect of corticosterone on antizyme mrna and protein expression in early regenerating rat liver

作　　者：马建敏[1] 宁黔冀[1,2] 李文娟[1] 索世英[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]省部共建细胞分化调控国家重点实验室,河南新乡453007

出　　处：《解剖学报》2009年第3期405-408,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30340042);河南省科技攻关资助项目(0624410108)

摘　　要：目的研究外源皮质酮对部分肝切除（PH）诱导的大鼠早期再生肝抗酶mRNA及蛋白水平的影响。方法PH前3d对成年雄性SD大鼠进行双侧肾上腺切除术,皮质酮悬浮于芝麻油中皮下注射去肾上腺鼠,抗酶mRNA及其蛋白水平的测定采用RT-PCR和Western blotting法。结果与对照组（n=6,以下相同）相比,去肾上腺组及10mg/kg、20mg/kg体重皮质酮处理组抗酶mRNA水平变化不明显,而40mg/kg体重皮质酮处理能显著刺激其转录。在PH后5、7、9h,去肾上腺术鼠抗酶蛋白水平显著低于相应时间点的对照组;皮质酮处理后,蛋白水平在所观察的整个实验过程中均显著上升,且剂量越高,蛋白含量越多,尤其在PH后5h,10、20和40mg/kg体重皮质酮处理组分别比同时间点对照组高43%、79%和93%。结论皮质酮对大鼠早期再生肝抗酶蛋白的合成具有剂量依赖性促进作用。Objective The effect of corticosterone on both antizyme mRNA and protein levels in early regenerating rat liver induced by partial hepatectomy （PH） was investigated. Methods Bilateral adrenalectomies （ADX） were performed 3 days before PH. Corticosterone in sesame oil was injected subcutaneously to ADX rats. Antizyme mRNA and its protein level were examined by RT-PCR and Western blotting respectively. Results When compared with control （ n = 6, the same below） group, there were no significant changes in antizyme mRNA levels in ADX, 10 and 20 mg/kg body weight corticosteronc-treated groups, whereas 40 mg/kg body weight corticosterone treatment markedly induced antizyme mRNA expression. Antizyme protein content in ADX group decreased significantly at 5, 7 and 9 hours after PH, however, it could be stimulated in a dose-dependent manner following corticosteronc administration during the whole experiment observed, particularly, at 5 hours post-PH antizymc protein levels in 10, 20 and 40 mg/kg body weight corticosterone-treated rats increased by 43%, 79% and 93% respectively, as compared with control group at the same time point. Conclusion Corticosteronc may dose-dependently induce antizyme protein synthesis in early regenerating rat liver; 10 or 20 mg/kg body weight corticosterone treatment has little effect on antizyme mRNA level, but 40 mg/kg body weight corticosterone stimulates antizyme gene transcription.

关 键 词：皮质酮 再生肝 抗酶 反转录-聚合酶连反应 免疫印迹法 大鼠 

分 类 号：Q71[生物学—分子生物学]