5-HT、生长抑素及P物质在海洛因依赖大鼠直肠的定位和表达  

Expression and localization of 5-HT,somatostatin and substance P of rectum in heroin dependent rats

作　　者：洪艳[1] 梁文妹[1] 刘霞[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室

出　　处：《解剖学报》2009年第3期463-468,共6页Acta Anatomica Sinica

基　　金：教育部“春晖计划”资助项目(Z2005-1-52020);贵州省优秀科技教育人才省长专项资金资助项目(黔科教办[2001-3]);贵州省科技攻关项目资助(黔科合S字[2007]1048)

摘　　要：目的探讨海洛因依赖期间大鼠直肠内5-HT、生长抑素（SS）和P物质（SP）免疫反应（IR）细胞的形态学改变。方法选取成年SD大鼠,分为海洛因依赖组（30只）、盐水对照组（30只）和正常对照组（6只）,皮下注射海洛因建立大鼠海洛因依赖模型。取直肠组织用免疫组织化学SABC法及图像分析法进行研究。结果与正常组及盐水对照组比较,海洛因依赖组大鼠直肠5-HT、SS及SP免疫反应细胞的细胞数均增多（P〈0.05）。5-HT、SS、SP-IR细胞免疫染色增强,图像分析显示,海洛因依赖期间大鼠直肠内5-HT、SS及SP免疫反应细胞的平均灰度值均低于正常组及盐水对照组（P〈0.05）;其中5-HT、SS-IR细胞以31d时间组最低（P〈0.05）,SP-IR细胞以38d时间组最低（P〈0.01）。结论海洛因依赖期间直肠5-HT、SS和SP-IR细胞的细胞数及平均灰度值均发生变化,提示5-HT、SS和SP合成和分泌增多。Objective To explore the morphological changes of 5-HT, Somatostatin （SS）, Substance P （SP） immunoreactive（IR） cells in rat rectum during heroin dependence period. Methods Normal SD rats were divided into normal control group （NCG）, saline control group（ SCG）, and heroin dependence group（ HDG）. To make the heroin dependence model of rats by subcutaneous injection of herion, and excise the rectum. Immunohistochemical SABC method, counting the number of cells and image analysis were used in the study. Results Compare with the NCG and SCG, All the number and immunohistochemical reaction of 5-HT, SS, SP-IR cells increased （ P 〈 0.05 ） in rectum during heroin dependence. The result of image analysis showed that the mean of grey degree of the rectum 5-HT, SS, SP-IR cells decreased in HCG as compared with the NCG and SCG（ P 〈 0.05） .And in 5-HT, SS-IR cells, the mean grey scale were the lowest on 31st day（ P 〈 0.05）. But in SP-IR cells, the mean grey scale was the lowest on 38th day（ P 〈 0.01 ）. Conclusion The changes manifested that the function of 5-HT, SS and SP-IR cells had enhanced synthesis and secretion during heroin dependence.

关 键 词：海洛因依赖 5-HT 生长抑素 P物质 直肠 免疫组织化学 大鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]