Bcl-2/Bax蛋白在生后大鼠卵巢不同发育时期的表达  

Expression of Bcl-2/Bax in postnatal rat ovaries at different developmental days

作　　者：屈丽华[1] 屈姣华 刘月顺[1] 龙治峰[1] 罗文奇[1] 石金凤[1] 罗红梅[1] 

机构地区：[1]南华大学医学院组织学胚胎学教研室,湖南衡阳421001 [2]广东省佛山市顺德区勒流镇医院妇产科,广东佛山528322

出　　处：《解剖学报》2009年第3期469-473,共5页Acta Anatomica Sinica

基　　金：湖南省教育厅基金资助项目(04C573)

摘　　要：目的探讨Bcl-2/Bax与卵泡发育和凋亡的关系。方法用免疫组织化学技术和图像分析系统,对95只大鼠生后不同发育时期卵巢中凋亡相关因子Bcl-2/Bax的表达情况进行研究。结果0-360 d卵巢各级卵泡中的卵母细胞均呈Bcl-2/Bax阳性染色。正常卵细胞中Bcl-2反应强于Bax,退化卵细胞中Bax的反应强于Bcl-2。Bcl-2/Bax在卵泡细胞中的表达模式有所不同,21d前Bcl-2为阳性,21-150d之间为Bcl-2阴性;180d以后正常卵泡为阴性,闭锁卵泡为阳性;Bax的表达,180d前全为阴性,180d后的表达同Bcl-2。膜细胞中Bcl-2/Bax的表达与卵泡细胞存在相似之处。Bcl-2/Bax在黄体中的表达只见于180d后。结论Bcl-2/Bax对卵细胞的生长、发育、存活和凋亡可能有重要的调节作用,其对卵泡细胞、膜细胞及黄体细胞的存活和凋亡的调节作用不明显。Objective To development and apoptosis. Methods investigate the relationship between the expression of Bcl-2/Bax protein and follicular technology was utilized to study the distribution and positive reaction intensity of apoptosis protein Bcl-2/Bax in 95 rat ovaries from different postnatal developmental days. Value of absorbance of Bcl-2/ Bax positive reaction in ovary sections was measured by the computer image analysis system. Results Positive staining for Bcl-2/ Bax existed in the oocytes of different class follicles from 0 day-old to 360 day-old rat ovaries. Bcl-2 positive reaction in health oocytes was stronger than Bax, whereas Bax positive reaction in degenerative oocytes was stronger than Bcl-2. The expression pattern of Bcl-2/Bax was different in follicular cells, in which Bcl-2 was positive reaction from the postnatal first 21days rat, negative reaction from 21 day-old to 150 day-old rat, and negative in health follicles and positive in atretic follicles from older rat than 180 day-old. Bax reaction in follicular ceils was all negative from younger rat than 180 day-old and liked Bcl-2 from older rat than 180 day-old. Expression of Bcl-2/Bax in theca cells was similar to follicular cells. Expression of Bcl-2/Bax in corpora luteum was observed only from older rat than 180 day-old. Conclusion Bcl-2/Bax may play an important role in regulating oocyte growth, development, survival and apoptosis. Their action on survival and apoptosis of follicular cells, theca cells and corpora luteum is not obvious.

关 键 词：卵泡 BCL-2 BAX 免疫组织化学 大鼠 

分 类 号：Q954.4[生物学—动物学]