C57小鼠胸腺和睾丸中mPer1基因启动子区CpGs甲基化分析  

Analysis of Methylation at Promoter of mPer1 Gene in C57 BL/6J Mouse Thymus and Testis

作　　者：李宁[1,2] 刘姝[2] 蔡彦宁[2] 陈彪[2] 

机构地区：[1]首都师范大学生命科学学院生殖与发育实验室,北京100048 [2]首都医科大学宣武医院神经生物室,北京100053

出　　处：《首都师范大学学报（自然科学版）》2009年第3期33-37,共5页Journal of Capital Normal University：Natural Science Edition

基　　金：国家自然科学基金(30400148;30430280;3042801040;30371574);科技部重点项目(2006CB500701;2004BA702B02);北京市自然科学基金项目(7031002);北京市科技新星计划(H020821400190)

摘　　要：利用实时定量PCR方法检测胸腺和睾丸组织中mPer1基因表达时相特点,采用亚硫氢酸修饰法对两种组织两个时间点该基因2个启动子区域5个E-box CpGs甲基化情况进行研究.结果显示:C57 BL/6J小鼠胸腺和睾丸组织中mPer1基因表达没有显著波动性,mPer1基因启动子区CpGs呈低甲基化状态(<10%).两种组织中E3和E4甲基化频率存在显著差异(P<0.01).在睾丸中,不同时间点E3和E4甲基化频率有显著变化(P<0.01).说明在睾丸中,mPer1启动子E-box3的甲基化状态能够随时间波动.但在胸腺和睾丸中,甲基化并不是mPer1基因调节的主要方式.Objectives： To investigated whether CpG methylation contributes to the atypical roPer1 transcription in testis and thymus .Methods： Real time PCR was employed to detect circadian expression of mPerl gene. Five E-box-containing amplicons were examined in mouse testis and thymus at both early-loght （Zeitgeber time 01, ZT01 ） and early-dark （ZT13） using bisulfite genomic sequencing. Results： E-boxes were sparsely methylated in testis and thymus at both times. Significant different of E3 and E4 were found between thymus and testits. Significant increases of methylation were observed at least in testis at ZT13. Conclusion： Methylation of E-box CpGs does not underly the basis of rnPerl atypical rhythmic expression in testis and thymus. Sub-groups of CpGs around E-boxes 3 and 4 appears to be preferentially methylated, and might play a role in regulating mPerl transcription.

关 键 词：roper1基因 启动子 CPG岛 甲基化 实时定量PCR 

分 类 号：Q234[生物学—细胞生物学]