磷素对怀牛膝耐铜毒性的影响研究  

Effect of phosphorus on copper tolerance in Achyranthes bidentata

作　　者：王新民[1,2] 陈士林[2] 魏志华[1] 介晓磊[1] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011 [2]中国医学科学院中国协和医科大学药用植物研究所,北京100193

出　　处：《中国中药杂志》2009年第12期1489-1492,共4页China Journal of Chinese Materia Medica

基　　金：河南省杰出青年基金项目(074100510018);中国博士后科学基金项目(20070410616)

摘　　要：目的:通过施磷(P)来调控怀牛膝对铜(Cu)的吸收和累积,防止怀牛膝过量吸收重金属Cu。方法:采用2因素完全设计,用自制PVC管栽培。结果与结论:Cu处理间、P处理间以及Cu,P交互间怀牛膝生物量差异都达到显著水平,土壤中P,Cu均为100 mg.kg-1时生物量最大;低Cu可以增加怀牛膝产量,Cu超过200 mg.kg-1后,怀牛膝产量、质量均下降,但对药材中活性成分齐墩果酸和脱皮甾酮的含量无影响。P的施入可以减轻高Cu对植株的毒害,怀牛膝产量增加,重金属Cu含量降低,但是当土壤Cu超过200 mg.kg-1后,怀牛膝药材中Cu高于20.0 mg.kg-1的限度,因此要生产出合格的怀牛膝药材,种植基地土壤中Cu一定要低于200 mg.kg-1。Objective： To study the effect of phosphorus on copper tolerance in Achyranthes bidentata. Method： A PVC pipe experiment was conducted to study the interactive effects of phosphorus （P） and copper （Cu）, on growth, elemental accumulation and chemical constituents of A. bidentata. Two levels of elemental P were applied at 0 （ P0 ） and 100 （ P100 ） mg .kg^ -1 soil with 5 levels of Cu at 0 （Cu0 ）, 100 （Cu100）, 200（Cu200 ）, 200（ Cu400 ）, 200（ Cu600 ）mg.kg^-1 soil, respectively. Result and Conclusion： The biomass production between different Cu treatments, phosphorus treatment showed significant differences. The biomass reached the maximum value as the concentration of Cu and P was 100 mg.kg^-1. Low concentration of Cu improved the growth ofA. bidentata. The growth was blocked as Cu concentration reached 200 mg.kg^- 1 in soil, however the contents of oleanolie acid and eedysterone in roots of A. bidentata had not influenced by Cu. P could improved the copper tolerance in A. bidentata and increased root yield. The Cu concentration in soil of the cultivation bases must be below 200 mg.kg^-1 in order to produce good quality of medicinal material.

关 键 词：怀牛膝 铜 磷 

分 类 号：R284[医药卫生—中药学]