清开灵注射液的抗血栓作用及其机制研究  

Anti-thrombosis effect and its mechanism of Qingkailing injection

作　　者：曹春雨[1] 梁爱华[1,2] 赵雍[1] 刘婷[1] 李春英[1] 易艳[1] 王金华[1] 薛宝云[1] 陈明伟[2] Odd G Nilsen 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]澳门科技大学

出　　处：《中国中药杂志》2009年第12期1549-1552,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(39870910,39970902);国家重大基础研究发展(973)计划(G1999054407,2006CB504700);国家科技支撑计划(2006BAI4B00)

摘　　要：目的:探讨清开灵注射液(QKL)的抗血栓作用及其机制。方法:将SD大鼠随机分为对照组、模型组、QKL 2.5,5.0,10.0 mL.kg-1组。QKL各剂量组均ip给药,每天1次×4 d。除正常对照组外,其余各组均于末次给予QKL前16 hip角叉菜胶(Ca)5 mg/只(0.5 mL)。于末次给予QKL后30 min,除了正常对照组iv生理盐水1 mL.kg-1外,其余各组均iv给予内毒素(LPS)50μg.kg-1(1 mL.kg-1),以注射LPS时作为造模0 h。于造模后2 h取血测定血浆TNF-α,IL-6含量和白细胞黏附分子CD11b/CD18表达;4,24 h测定外周血白细胞和血小板数量和测量尾部血栓长度(cm);120 h测定血小板聚集率。结果:清开灵注射液可明显抑制血栓形成,缩短尾部血栓长度;明显抑制体内炎性因子TNF-α和IL-6产生,降低白细胞黏附分子CD11b/CD18的表达水平;减轻由于血栓形成而导致的血小板消耗;降低血小板聚集率。结论:清开灵注射液的抗血栓作用与其抑制白细胞的激活和黏附,降低炎症反应和炎性因子产生,抑制血小板聚集等有关,这些作用机制与其清热解毒功效一致。Objective： To investigate the anti-thrombosis effect and its mechanism of Qingkailing injection （QKL）. Method ： SD rats were randomly divided into control group, model group and QKL 2. 5, 5.0, 10 groups. QKL were given （ip） to rats once a day for successively 4 days. The rats in all groups but control were pretreated with carrageenin （Ca） ip at 16 h before the last dose of QKL and followed by intravenous injection of endotoxin （ LPS fore E. coli Olll ：B4） 50 μg .kg^-1 30 min after the last dosing of QKL. Thrombosis in rat tails were observed at 24 h after injection of LPS. The number of white blood cells and platelets, serum TNF-α, IL- 6 level, CD11b/CD18 expression of white blood ceils and platelet aggregation were analysed. Result： QKL obviously inhibited the LPS/Ca-induced thrombosis as showed a reduced infarction range due to thrombosis in tails. The sera concentration of TNF-α and IL- 6, expression of CD11 b/CD18 in WBC and platelet coagulation rate were reduced after QKL treatment. Conclusion： The anti-thrombosis action of QKL is associated with inhibition of WBC activation and adherence, reduction of inflammatory factor release and abating of platelet coagulation rate. The anti-thrombosis mechanism of QKL is consistent with its function of clearing away heat-evil and toxic materials.

关 键 词：清开灵注射液 清热解毒 血栓 炎症 

分 类 号：R285.5[医药卫生—中药学]