犀角地黄汤对大鼠脑出血急性期Bcl-2,caspase-3,TNF-α,IL-6表达的影响  

Effects of Xijiao Dihuang decoction on the expressions of Bcl-2,caspase-3,TNF-α and IL-6 after acute intracerebral hemorrhage in rats

作　　者：秦峰[1] 王长松[1] 晋光荣[2] 花菊兰[2] 

机构地区：[1]东南大学临床医学院,江苏南京210009 [2]东南大学基础医学院,江苏南京210009

出　　处：《中国中药杂志》2009年第12期1566-1569,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2006CB504807)

摘　　要：目的:观察犀角地黄汤对大鼠脑出血急性期脑组织Bc l-2、半胱氨酰天冬氨酸特异性蛋白酶-3(caspase-3)、血肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达的影响。方法:以自体血制作大鼠脑出血模型,采用免疫组织化学和放射免疫法检测大鼠脑出血急性期脑组织Bc l-2,caspase-3阳性细胞数,血TNF-,αIL-6的表达。结果:造模后72 h,模型组caspase-3阳性细胞数、TNF-,αIL-6的表达明显高于正常组、假手术组;犀角地黄汤各剂量组caspase-3阳性细胞数、TNF-α及IL-6的表达明显低于模型组,Bc l-2阳性细胞数明显高于模型组。结论:犀角地黄汤可能通过减少细胞凋亡、抑制炎症,对脑出血后的继发性神经元损伤起保护作用。Objective： To study the effects of xijiao dihuang decoction on the expressions of Bcl-2 and caspase-3 protein positive ceils in brain tissue, TNF-α and IL-6 in blood after acute intracerebral hemorrhage in rat. Method： The experimental ICH model was established by injection of non-heparinized autologous arterial blood into the right basal ganglia. Immunohistochemistry and radio- immunity methods were used to detect expressions of Bcl-2 and caspase-3 protein positive cells in brain tissue, TNF-α and IL-6 in blood. Result： After ICH, caspase-3 protein positive cells in brain tissue, TNF-α and IL-6 in blood increased obviously in the model group, caspase-3 protein positive cells, TNF-α and IL-6 were significantly reduced by treatment with Xijiao Dihuang decoction, on the contrary, Bcl-2 protein positive cells in brain tissue increased markedly. Conclusion： Xijiao Dihuang decoction can pretect nerve cell by reducing the expression of caspase-3, TNF-α and IL-6,increasing the expression of Bcl-2 after ICH in hemorrhagic brain damage.

关 键 词：犀角地黄汤 脑出血 大鼠Bcl-2 caspase-3 TNF- αIL-6 

分 类 号：R285.5[医药卫生—中药学]