拉伸应变对骨髓间充质干细胞成骨细胞定向分化的影响  

Effect of Mechanical Strain on Differentiation of Mesenchymal Stem Cells into Osteoblasts

作　　者：赵红斌[1] 周焕发[1] 王先华[1] 董菊子[1] 杨银书[1] 张西正[2] 

机构地区：[1]兰州军区兰州总医院,兰州730050 [2]军事医学科学院卫生装备研究所,天津300161

出　　处：《生物医学工程学杂志》2009年第3期518-522,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30470438);军事医学科学院创新基金项目资助(CX-04036)

摘　　要：探讨拉伸应变对小鼠骨髓间充质干细胞(Mesenchymal stemcells,MSCs)向成骨细胞定向分化的影响。在成骨细胞诱导条件下,利用免疫组织化学和RT-PCR技术,(1)探讨0.5 Hz,3%拉伸应变作用MSCs不同时间(15 min、30 min、1 h、2 h、4 h、3 d、7 d、14 d),碱性磷酸酶(Alkaline phosphatase,ALP)、Ⅰ型胶原(TypeⅠcollagen,COLI)蛋白表达的变化以及Osterx(Osx)和骨钙素(Osteocalcin,OCN)mRNA表达的变化;(2)探讨不同拉伸应变(1%、3%和10%)作用1 h,Osx mRNA和OCN mRNA表达的变化。结果表明:(1)3%拉伸应变作用7 d,ALP的表达最强,14 d ALP表达减弱;3 d COLI表达同对照组相比显著增加;7 d、14 d COL I表达明显增强。(2)MSCs成骨诱导15 min,Osx mRNA开始表达,2 h Osx mRNA表达增加。0.5 Hz,3%应变作用MSCs 15 min,Osx mR-NA表达明显增加;30 min和1 h Osx mRNA的表达同成骨诱导组相比显著增强。成骨诱导15 min,OCN mRNA未见表达,作用30 min;OCN mRNA表达丰度低;1 h OCN mRNA表达升高。0.5 Hz,3%应变作用MSCs 15min,OCN mRNA开始表达;作用30 min和1 h,OCN mRNA表达明显增加。(3)1%和3%应变作用MSCs 1 h,OsxmRNA的表达显著上调;10%应变作用MSCs 1 h,Osx mRNA的表达不显著。3%的应变明显上调OCN mRNA的表达,1%和10%应变对OCN mRNA表达影响较小。提示,一定范围内的应变能促进小鼠骨髓间充质干细胞向成骨细胞定向分化的进程。This study sought to elucidate the effect of mechanical strain on the differentiation of mesenchymal stem cells into osteoblasts. Under the conditons of inducing osteoblasts, Immunohistochemical methods and RT-PCR technology were applied in osteogenic supplements medium to detect. （1） the expression of Alkaline phosphatase （ALP）, Type Ⅰ collagen （COL Ⅰ）, Osterx（Osx） and Osteocalcin （OCN） mRNA, with cyclic strain（3%, 0. 5 Hz） applied for 15 min,30 min,1 h,2 h,4 h,3 d,7 d,14 d; （2）the expression of Osx mRNA and OCN mRNA with 3% strain for 1 h. The results showed.. （1） ALP mRNA expression was higher at 7 days; COL I mRNA expression was greater obviously at 7 days and 14 days than that at 3 days and that of the unstrained cells; （2） the expression of Osx mRNA was up-regulated after 15min by strain stimulation,which was significantly increased at 30 min and 1 h in the unstrained cells. The expression of OCN mRNA was not affected in the unstrained cells at 15 min, whereas strain could promote the expression of OCN mRNA at this period. The expression of OCN mRNA was more obviously upregulated in the strained cells at 30 min and 1 h when compared with that in the unstrained cells; （3） the strain （1% and 3%） significantly promoted the expression of Osx mRNA; 10% strain had a little effect on Osx mRNA expression. The expression of OCN mRNA was up-regulated by 3% strain, whereas it had little effect at 1% and 10% strain. In summary, mechanical strain can promote the differentiation of mesenchymal stem cells into osteoblasts.

关 键 词：应变 间充质干细胞 成骨细胞 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]