日本血吸虫紫外线致弱尾蚴免疫鼠诱导的抗病免疫效应  

Anti-schistosomiasis effect induced by UV-attenuated cercariae of Schistosoma japonicum in C57BL/6 mice

作　　者：陈家旭[1] 刘述先[1] 徐馀信[1] 宋光承[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病与丝虫病合作中心,上海200025

出　　处：《中国血吸虫病防治杂志》2009年第3期179-182,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家科技支撑计划项目(2008BAI56B03)

摘　　要：目的观察日本血吸虫紫外线致弱尾蚴(UVC)疫苗免疫小鼠诱导的抗肝虫卵肉芽肿及纤维化效应。方法将60只C57BL/6小鼠随机分为UVC疫苗免疫组和感染对照组。疫苗免疫组小鼠经皮肤接种UVC后5周,每鼠攻击感染(30±2)条正常日本血吸虫尾蚴;感染对照组经皮肤感染同量尾蚴。于攻击感染后7周解剖小鼠;取肝左叶制备连续石蜡切片,测定肝脏单卵肉芽肿大小;用ELISA法检测血清透明质酸(HA)及层黏连蛋白(LN)含量,PCR-ELISA法检测肝组织TGF-β1mRNA的表达水平。结果UVC疫苗免疫组小鼠肝组织单卵肉芽肿直径为(176.25±38.67)μm,显著小于感染对照组的(304.38±53.23)μm(P<0.01),与感染对照组相比,UVC疫苗免疫组小鼠肝虫卵肉芽肿直径减小了42.10%。UVC疫苗组小鼠血清中HA、LN含量均显著低于感染对照组,肝纤维化程度明显减轻。结论UVC疫苗免疫小鼠诱导的抗肝虫卵肉芽肿及其纤维化效应同疫苗免疫诱导的细胞免疫应答的增强及高水平的IFN-γ以及肝TGF-β1mRNA表达水平的降低密切相关。Objective To observe the anti-schistosomiasis egg granuloma and hepatic fibrosis effects in C57BL/6 mice immunized with UV-attenuated cercariae （UVC） vaccine. Methods Sixty C57BL/6 mice were divided into two groups, a UVC group and infection control group. The mice of the UVC group were challenged with cercariae of Schistosoma japonicum five weeks after immunizing with UVC. The mice in the infection control group were challenged with the same number of cercariae. The mice were sacrificed 7 weeks after the challenge infection to get the left livers. The consecutive paraffin sections of the livers were made and stained with haematoxylin and eosin, and the size of single egg granulomas was measured with micrometer. The levels of hyaluronic acid （HA） and laminin （LN） in sera of the mice were determined by ELISA. The expression of TGF-β1mRNA of the livers were examined by PCR-ELISA. Results The mean hepatic egg granuloma diameters of the two experimental groups, the UVC group and infection control group, were （ 176.25 ±38.67）μm and （304.38±53.23）μm, respectively. The hepatic granulomas were significantly smaller in the UVC group than those in the infection control group. The liver granuloma size of mice in the UVC group was decreased by 42.10% in diameter as compared with the infection control group. The levels of HA and LN in sera of mice of the UVC group were significantly lower than those of the infection control group （P 〈 0.01 ）. The amount of TGF- β1mRNA isolated from the livers of mice in the UVC group decreased significantly. Conclusions UVC vaccine acts as an effective inhibitor against egg granulomas of Schistosoma japonicum and reduces immunopathological damage caused by Schistosoma japonicum in the host.

关 键 词：日本血吸虫 紫外线致弱尾蚴 虫卵肉芽肿 透明质酸 层黏连蛋白 TGF-Β1 mRNA 

分 类 号：R383.34[医药卫生—医学寄生虫学]