绿茶多酚对小鼠皮肤伤口愈合时白介素-1β表达的影响  

EFFECTS OF(-)-EPIGALLOCATECHIN-3-GALLATE ON INTERLEUKIN-1β EXPRESSION IN MOUSE WOUND HEALING

作　　者：申羽佳[1] 李卫国[1] 张惠[1] 李宾[1] 袁红旭[1] 张江江[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《分子细胞生物学报》2009年第3期179-185,共7页Journal of Molecular Cell Biology

基　　金：河南省科技攻关计划(No.0624410029);新乡市科技发展计划项目(No.08S044);河南省重点学科基金

摘　　要：表没食子儿茶素-3-没食子酸酯[(-)-epigallocatechin-3-gallate,EGCG]是绿茶多酚的主要成分,具有抗氧化、抗衰老、抗肿瘤、抗炎症和杀菌等多种生物学效应。本文通过组织学、ELISA和RT-PCR技术研究了EGCG对皮肤伤口愈合期间损伤部位白介素-1β(interleukin-1β,IL-1β)基因表达及IL-1β分泌水平的影响效应。结果表明EGCG(50mg/L)处理72h后损伤部位的IL-1β基因表达受到抑制、IL-1β含量下降,而早期(24h内)EGCG(50mg/L)处理对IL-1β基因表达以及IL-1β的含量没有显著性影响。（-）-Epigallocatechin-3-gallate （EGCG）, a main constituent of green tea polyphenols, plays important roles in antioxidative, anti-senescence, anti-tumor, anti-inflammation and bacterail action. In this study, effects of EGCG on the expression of IL-1β gene and the IL-1β level at wound sites during cutaneous wound healing were investigated with histological stain, ELISA and RT-PCR technique. The results show that EGCG （50mg/L） inhibited the expression of IL-1β gene and reduced the IL-1β level at wound sites at 72 h after cutaneous wound, but not change the expression of IL-1β gene and the IL-1β level at early stage （≤24 h）.

关 键 词：没食子儿茶素-3-没食子酸酯 伤口愈合 白介素-1Β 

分 类 号：R285.5[医药卫生—中药学]