口服草原兔尾鼠卵透明带3纳米乳剂DNA疫苗免疫效果研究  

ORAL IMMUNIZATION OF LUGURUS LUGURUS WITH THE PLASMID NANOEMULSION OF ZONE PELLUCIDA 3 TO ENHANCE INFERTILITY EFFECTS

作　　者：张爱莲[1] 庄淑珍[1] 吴道澄[2] 李轶杰[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]西安交通大学生命科学与技术学院,西安710049

出　　处：《分子细胞生物学报》2009年第3期186-192,共7页Journal of Molecular Cell Biology

基　　金：国家自然科学基金(No.30760136);新疆大学青年教师科研启动金项目(No.QN040119)资助

摘　　要：用免疫不育疫苗控制有害动物的数量成为动物种群数量控制的一个有效措施,对于大多数动物来说,口服饵料发送系统是最好的选择。但是口服疫苗容易产生耐受,且生物利用度低,利用多聚物包裹口服疫苗是一种打破口服耐受,提高其生物利用度的新途径。本文以纳米乳剂作为草原兔尾鼠卵透明带3(Lugurus zone pellucida 3,LZP3)DNA疫苗的发送载体,探讨通过口服途径增强小鼠的免疫效果和抗生育率。用纳米乳剂包裹lzp3DNA疫苗后,采用强阴离子交换色谱法测定纳米乳剂的包封率,将制备好的质粒纳米乳剂lzp3 DNA疫苗通过口服饵料免疫小鼠,检测到免疫后小鼠的体内产生了特异性的抗LZP3的IgG和IgA。对免疫后小鼠进行抗生育实验,分析生育后免疫小鼠的卵巢病理切片。研究结果表明,用质粒纳米乳剂lzp3 DNA疫苗通过口服途径免疫,在小鼠的血清和粘液中检测到了特异性的抗LZP3的IgG和IgA,同时也产生了相应的抗生育作用,表明纳米乳剂作为口服不育疫苗的发送载体是可行的,为草原兔尾鼠鼠害的防治提供了理论基础。Immunocontraceptive technology is beginning to attract more attention for the control of wild mouse or other pest animals. Oral vaccination of animals and man, to provide effective mucosal and/or systemic immunity, is a good delivery system, but it remains poor bioavailablity, one of the most promising strategies to improve the oral delivery relies on polymeric nanoparticles carriers to encapsulate vaccine. Nanoemulsion was used as the carriers of oral immunization of Lugurus Lugurus zona pellucida 3, the immune response and antifertility potential of this vaccine is valuated. Nanoemulsion-encapsulated plasmid pcDNA3.0-Aat-COMP-lzp3-C3d3 （pACLC DNA vaccine） was prepared and evaluated the encapsulation efficiency by anion exchange chromatography. The mice were orally delivered with nanoemulsion-encapsulated plasmid, to detect specific IgG in serum and IgA in vaginal fluid by indirect ELISA, and to perform fertility experiment, to do histological analysis of immunized mice ovary. The results showed that the specific IgG and IgA antibody is elicited, the fertility rate is deceased at a certain extent. In conclusion, it is feasible that nanoemulsion is used as the carriers of oral immunization.

关 键 词：草原兔尾鼠 卵透明带3 纳米乳剂 口服DNA疫苗 免疫不育 

分 类 号：S812.6[农业科学—草业科学]