改进DNS比色法测定玉米芯水解液的总糖含量  

MODIFIED 3,5-DINITROSALICYCLIC ACID METHOD FOR DETERMINATION OF TOTAL SUGAR OF CORNCOB HYDROLYSATE

作　　者：姜岷[1] 姚嘉旻[1] 陈可泉[1] 吴昊[1] 谢鑫[1] 

机构地区：[1]南京工业大学生物与制药工程学院,材料化学工程国家重点实验室,南京210009

出　　处：《太阳能学报》2009年第6期820-823,共4页Acta Energiae Solaris Sinica

基　　金：国家高技术研究发展计划(863)项目(2006AA02Z235);国家自然科学基金(20606017);国家自然科学基金重点项目(20336010);江苏省高校自然科学研究项目(05KJB180043)

摘　　要：采用分别测定己糖和戊糖含量的方法对DNS比色法进行了改进。结果表明:采用改进DNS比色法测定玉米芯水解液中总糖含量的回收率在98.90%～100.81%,RSD=0.62%,可准确、快速、简便地测定玉米芯水解液中的混合总糖含量。The measuring precision of pentoses or hexoses by DNS method was well, but the measuring error of mixing total sugar of pentoses and hexoses was notable. Therefore, 3, 5-Dinitrosalicyclic acid method was modified by measuring of pentoses and hexoses respectively. The result showed the recollection rates of total sugar of corncob hydrolysate were in the range of 98.90% - 100.81%, the relative standard deviation was 0.62% and the precision in determination of mix- ing total sugar was raised obviously. So the method was accurate, fast and convenient and it was fit to determine total sugar in corncob hydrolysate in quantitation.

关 键 词：玉米芯 总糖 改进DNS比色法 定量测定 

分 类 号：TK511.4[动力工程及工程热物理—热能工程]