基于水解活性的Zn^(2+)荧光探针(英文)  

Hydrolysis-activated Fluorophore System as a Molecular Sensor for Selective Detection of Zn^(2+)

作　　者：吴红梅[1] 何成[1] 王健[1] 周硼[1] 

机构地区：[1]大连理工大学化学系精细化工国家重点实验室,辽宁大连116012

出　　处：《发光学报》2009年第3期275-284,共10页Chinese Journal of Luminescence

基　　金：National Natural Science Foundation of China(20801018);the Start-Up Fund of the Dalian University of Technology(893306)~~

摘　　要：合成了一种新颖的锌离子荧光探针N′(2-羟基苄基)-4-(2-羟基苯亚甲基胺)-苯甲酰肼(HHB),该探针本身的荧光较弱,但当它与Zn2+配位时荧光增强,在475nm处产生一较宽的发射光谱,量子产率f=0.59(λex=370nm)。该化合物对Zn2+具有良好的选择性能,环境生物体系中大量存在的碱金属和碱土金属离子K+,Na+,Mg2+和Ca2+以及过渡金属离子Mn2+,Co2+,Ni2+和Cu2+等对Zn2+的检测没有明显影响。在生命体系的pH值范围(6.8～7.7),配合物HHB显示出对Zn2+检测较高的灵敏度。作为比较文中还研究了HHB水解的主要产物4-氨基苄腙-水杨醛(HB)对Zn2+的识别与传感性能。A new selective Zn^2＋ fluorescent sensor, N＇- （2-hydroxybenzylidene） -4- （2-hydroxy-benzylidene-amino） benzohydrazide （HHB）, was synthesized. With the harbored 2-hydroxybenzylidene group, HHB exhibits an emission band centered at 475 nm with high quantum yield （f = 0.59） upon the addition of zinc ions. It features excellent fluorescence enhancement and provides favorable sensitivity for Zn^2＋ detection under the biological pH window of 6.8 - 7.7, and high selectivity for Zn^2＋ over biologically relevant alkali metals, alkaline earth metals and some of the first row transition metal. It works through the metal-assisted hydrolysis mechanism, and the hydrolysis residue sensor HB, salicylaldehyde-4-aminobenzoylhydrazone, was also investigated for comparison.

关 键 词：荧光传感器 Zn2+ 水解 选择性 

分 类 号：O482.31[理学—固体物理]