新生儿脐动脉用于血管移植材料的去抗原与抗钙化研究  

The experimental study on neutralization of antigenicity and decalcification of infant's umbilical artery as vascular grafts in coronary artery bypass grafting

作　　者：陈伟光[1] 陈耀新[1] 钟绍涛[1] 

机构地区：[1]暨南大学医学院第四附属医院(广州市红十字会医院),广东广州510220

出　　处：《中国热带医学》2009年第7期1228-1229,1234,共3页China Tropical Medicine

基　　金：广东省卫生厅立项课题(WSTJJ20061225440102196402113233)

摘　　要：目的探讨新生儿脐动脉用于冠状动脉架桥血管移植材料时去抗原性和抗钙化的较佳处理方式。方法采用目前常规三种去抗原性方法分别处理脐动脉,后采用多聚环氧化合物(EC)行抗钙化处理;检测各种方法处理后血管的去抗原性与抗钙化效果。结果冻干辐照+EC消除血管抗原性满意,但包理实验显示脐动脉钙化现象随时间延长而逐渐加强;戊二醛+EC组血管免疫原性明显减弱,且包理实验显示钙化情况最轻,但血管材料质地变硬,血管管腔减小,管壁增厚,缝合困难并易撕裂;液氮深低温保存60d+EC处理后的脐动脉免疫原性亦明显降低,同时包理实验显示脐动脉钙化现象较轻。结论采用冻干十辐照+EC,液氮深低温+EC,戊二醛+EC等方法处理脐动脉后均可在不同程度上达到去抗原、抗钙化效果,而采用液氮深低温+EC方法处理脐动脉的效果较好,容易实施,且血管可较长时间保存。Objective To discuss the neutralization of antigenicity and decalcification of infant＇s umbilical artery （UA） as vascular grafts in coronary artery bypass grafting. Methods The antgenicity of UA was neutralized by routine three methods respectively and decalcificated by epichlorohydrin （EC）. Results The results of neutralization of antigenicity and decalcification of impaired blood vessels by lyophilize-Irradiation and via EC fixation was satisfactory. The calcification of blood vessels would get stronger gradually along with the elongation of embedding time. In the group of glutaraldehyde-tanned vessel and via EC fixation （GA group）,vascular antigenicity decreased obviously. The degree of calcified vessel was the most gently among the three groups, but the vascular diameter decreased, the texture turned to rigid, the wall of vessels thickened and it was diflqcult to suture,easy to be tom. The antigenicity of Umbilical artery vessel cympreserved in liquid nitrogen for a period of 60 days and via EC fixation was reduced. Conclusions The results of neutralization of antigenicity and decalcification of UA can be achieved after dealed with lyophilize-Irradiation, cyropreserved in iquid nitrogen, tanned by glutaraldehyde respectively and via EC fixation.

关 键 词：脐动脉 同种异体血管材料 去抗原性 抗钙化 

分 类 号：R622.4[医药卫生—整形外科]