高血压并动脉硬化ACE基因多态性与危险因子的相关性  

Correlation ACE the gene polymorphisms and the risk factor of hypertension with atherosclerosis in Hainan Li Nationality

作　　者：徐波[1] 云美玲[2] 郑茵[3] 曾渝[2] 张勇[4] 金水晶[4] 王镇[4] 周代锋[2] 王利[2] 蔡望伟[2] 刘裕芬[3] 吴恳[4] 王小虹[2] 

机构地区：[1]海南边防总队医院,海南海口570208 [2]海南医学院附属医院心内科,海南海口570102 [3]海南省人民医院,海南海口570311 [4]海南省老年病医院,海南海口571100

出　　处：《中国热带医学》2009年第7期1235-1237,共3页China Tropical Medicine

基　　金：海南省自然科学基金项目(80586);海南省教育厅重点科研资助项目(HjKj2008-43)

摘　　要：目的探讨血管紧张素转换酶(ACE)基因多态性与高血压并动脉硬化危险因子的相关性。方法采用聚合酶链反应(PCR)检测260例海南黎族高血压并动脉硬化患者及276例黎族正常人的ACE基因插入/缺失(I/D)多态性,观察DD、DI、II基因型频率及等位基因频率。用高分辨超声技术分别检测DD、DI、II3个亚组的平均颈动脉内-中膜厚度(MIMT)。结果(1)高血压并动脉硬化组DD、DI、II基因型频率为15.0%、37.3%、47.7%;D及I等位基因频率分别为33.7%、66.3%。正常对照组DD、DI、II基因型频率17.8%、40.6%、41.6%,D及I等位基因频率分别为38.0%、62.0%。两组之间DD、DI、II基因型频率及D、I等位基因频率无显著性差异(P>0.05)。(2)高血压并动脉硬化组在胆固醇(TC)、甘油三酯(TG)、载脂蛋白A(ApoA)、载脂蛋白B(ApoB)、收缩压(SBP)、舒张压(DBP)显著高于对照组(P<0.05),而高密度脂蛋白(HDLC)显著低于对照组(P<0.05);Logistic回归分析结果显示,TG(OR=2.14)、ApoA(OR=360.39)、SBP(OR=1.21)、DBP(OR=1.08)、ACEDD基因型(OR=0.30)与高血压并动脉硬化病存在联系(P<0.05)。ACEDD型亚组的MIMT比DI和II型亚组显著增高(P<0.05)。结论ACE基因型缺失型具有颈动脉硬化易感性,可能是海南黎族高血压并动脉硬化的危险因素,可作为动脉粥样硬化的一个早期预测因子。Objective To study the correlation between the angiotensin-converting enzyme （ACE） gene polymorphisms and the risk factor of hypertension with atherosclerosis. Methods ACE I/D gene polymorphisms were detected by polymerase chain reaction （PCR） in 260 hypertension with atherosclerosis group and 276 healthy group from Hainan Li nationality,and investigated the genotype frequencies and allele frequencies of DD,DI and Ⅱ. Used high-resolution ultrasound techniques to detect mean intina-media thickness （MIMT）in DD, D Ⅰ and Ⅱ subset of people. Results （1） In the hypertension with atherosclerosis group of Hainan Li nationality,the genotype frequencies of DD,DI and Ⅱ were 15.0%, 27.3%,47.7% respectively,and the allele frequencies of D and Ⅰ were 33.7% and 66.3% respectively. In the healthy control of Hainan Li nationality,the genotype frequencies of DD,DI and Ⅱ were 17.8% ,40.6% and 41.6% respectively,and the allele frequencies of D and Ⅰ were 38.0% and 62.0% respectively. There were no significant differences beth in the genotype frequencies of DD,DI and Ⅱ,and in allele frequencies D and I between these two groups （P〉0.05）. （2） In all hypertensive patients,the MIMT level in ACE DD subset were significant higher than in DI and Ⅱ （P〈0.05）. In the hypertensive grade 1 and grade 3 group,the MIMT level in ACE DD subset were significant higher than in DI and Ⅱ （P〈0.05） respectively. There were no significant differences between the two groups （P〉0.05）. The total serum cholesterol （TC）,triglyceride（TG）, apolipoprotein A （ApoA）,apolipoprotein B （ApoB）,systolie pressure （SBP） and diastolic pressure （DBP） level in the hypertension with atheroosclerosis group were significantly higher than in the control （P〈0.05）.The high density lipoprotein cholesterol （HDL-C） level in the hypertension with atherosclerosis was significant lower than the control （P〈0.05）. The logistic regression showed that there were associations between TG （O

关 键 词：血管紧张素转换酶(ACE) 基因多态性 黎族 高血压 动脉硬化 危险因子 

分 类 号：R544.1[医药卫生—心血管疾病]