大孔吸附树脂对白簕叶总皂苷的纯化工艺研究  

Studies on Purification of Total Saponins from the Leaves of Acanthopanan trifoliatus by Macroporous Adsorption Resin

作　　者：高侠[1] 黎云祥[1] 蔡凌云[1,2] 王荣[1] 权秋梅[1] 陈蕉[1] 

机构地区：[1]西华师范大学生命科学学院/四川省高校环境科学与生物多样性保护重点实验室/教育部重点实验室,四川南充637002 [2]凯里学院化学系,贵州凯里556003

出　　处：《中药材》2009年第5期780-784,共5页Journal of Chinese Medicinal Materials

基　　金：四川省教育厅重点项目(2006A078);四川省重点学科项目(SZD0420)

摘　　要：目的:研究大孔吸附树脂富集纯化白簕叶总皂苷。方法:以白簕叶总皂苷产率为评价指标,考察静态吸附量和解吸率筛选出最适型号树脂,再对上样pH值、动态吸附流速、最佳上样量、洗脱剂浓度等纯化工艺条件参数进行研究。结果:大孔吸附树脂法纯化白簕叶总皂苷的最优条件为:选用AB-8型大孔吸附树脂,吸附液pH值为5～6,吸附流速为1.0mL/min,吸附液用量与树脂体积比为4∶1,洗脱剂为70%乙醇,洗脱剂用量与树脂体积比为4∶1,解吸流速为2.0mL/min。Objective ： To study the enrichment and purification of total saponins from the leaves of A. trifoliatus. Methods ： Using the total saponins from the leaves of A. trifoliantus as the standard index, we examinad the capability of static adsorption and desorption to select the optimum macroporous adsportion resin. Then we studied the purification technological parameters such as pH value, the dvnamic adorption speed of flow, the best type quantity, the eluent density and so on. Results： The optimum purification conditions were as follows ： the type of macroporous adsorption resin was AB-8, the pH value of absorbing solution was 5 - 6, the absorbing velocity of flow was 1.0 mL/min, the concentration of ethanol as elutent was 70% , the proportion of the amount of absorbing solution and resins was 4： 1, the proportion of the amount of elutent and resins was 4： 1, the eluting velocity of flow was 2.0 mL/min.

关 键 词：白筋 吸附 解吸 皂苷 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]