维生素E琥珀酸酯联合5-氟尿嘧啶抗人肝癌细胞增殖作用的研究  

The anti-proliferation effect of vitamin E succinate combined with 5-fluorouracil on human liver cancer cells

作　　者：刘涛[1] 孔连宝[1] 张业伟[1] 宋德静[1] 袁景伦[1] 

机构地区：[1]南京医科大学第一附属医院肝脏外科,江苏南京210029

出　　处：《中国生化药物杂志》2009年第3期158-161,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：江苏省"兴卫工程"重点人才基金资助项目(项目编号:rc2007058)

摘　　要：目的观察维生素E琥珀酸酯(VES)联合5-氟尿嘧啶(5-FU)抗人肝癌细胞SMMC-7721的增殖作用。方法体外培养的SMMC-7721细胞以VES联合5-FU分别作用24和48h,MTT法测定细胞增殖的抑制作用;流式细胞仪分析细胞周期、凋亡率以及Fas的表达。结果MTT检测显示,VES6μg/mL作用细胞24h后抑制率为(2.78±0.05)%,随药物浓度的增加和作用时间的延长,抑制率显著增加(P<0.01);相同条件下,VES与5-FU合用较两药单用抑制作用显著增加(P<0.01)。流式细胞仪分析显示,SMMC-7721细胞自然凋亡率为(0.86±0.20)%,VES24μg/mL,5-FU30μg/mL及两者合用作用细胞24h后凋亡率分别为(30.08±2.32)%,(18.23±1.58)%和(63.68±2.88)%(P<0.01),细胞表面Fas表达增加。结论VES联合5-FU通过阻滞细胞周期于G0/G1期、促进细胞表面Fas的表达协同抑制肝癌细胞增殖。Purpose To observe the anti-proliferation effect of vitamin E succinate （ VES ） combined with 5-fluorouracil（5-FU） on human liver cancer cells. Methods SMMC-7721 liver cancer cells cultured in vitro were treated with VES combined with 5-FU for 24 h and 48 h. The inhibitory effect was measured by MTT method, and the cell cycle, apoptotic rate and cell surface Fas expression were analyzed by flow cytometry assay. Results MTT indicated that the inhibitory rate was （2.78 ± 0.05） % in VES 6 μg/mL treatment group after 24 h, and the effect was dependently related to time and dosage（ P 〈 0.01 ）, the combined group had significant inhibitory effect on the growth of human liver cancer cells（ P 〈 0.01 ）. Flow cytometry assay showed that the natural apoptotic rate of SMMC-7721 cells was （0.86 ± 0.20） % .After treatment with VES 24 μg/mL, 5- FU 30 μg/mL,and VES 24 μg/mL combined with 5-FU 30 μg/mL for 24 h,the apoptotic rates were （30.08 ± 2.32） %, （ 18.23 ± 1.58） % and （63.68 ± 2.88） % （ P 〈 0.01 ）, respectively. Fas expression increased after the co-administration of VES and 5-FU. Conclusion VES combined with 5-FU had synergistic inhibition effect on human SMMC-7721 liver cancer cells. The mechanism included the cell cycle blockage at the G0/G1 phase and the increase of Fas expression on the surface of liver cancer cells.

关 键 词：维生素E琥珀酸酯 5-氟尿嘧啶 肝癌细胞 凋亡 

分 类 号：R965[医药卫生—药理学]