毛细管电泳法测定宁心宝胶囊中的核苷类成分  

Determination of nucleosines in Ningxinbao Capsules by CE

作　　者：姜雯[1] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023

出　　处：《中国生化药物杂志》2009年第3期187-189,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的建立毛细管电泳法测定宁心宝胶囊中核苷类成分含量的方法。方法采用Agilent毛细管电泳仪,熔融石英毛细管56cm×75μm,有效长度50cm,自动压力(35mPa)进样3s,检测波长254nm,分离电压20kV,运行缓冲液为36mmol/L硼砂溶液-15mmol/L磷酸二氢钠溶液(pH9.2)。结果宁心宝胶囊中主要成分腺苷、鸟苷、尿苷、肌苷分别在10.1～252.5,10.3～258.0,10.3～257.6,10.8～270.0mg/L范围内有良好的线性关系,r分别为0.9998,0.9997,0.9996,0.9995。加样回收率分别为98.4%,102.5%,97.2%,101.3%。RSD分别为2.2%,1.8%,2.6%,2.0%。结论此法简便、快速,适用于宁心宝胶囊中核苷类成分的测定,为控制其质量提供了简便、易行的方法。Purpose To establish CE method for the assay of nucleosines in Ningxinbao Capsules. Meth- otis Agilent apparatus equipped with a UV detector and a untreated fused-quartz capillary（56 cm × 75 μm,50 cm effective length）were used. Pressure injection was 35 mPa for 3 s,and the wavelength of detector was 254 nm.20 KV was applied for separation. The running buffer was 36 mmol/L Sodium borate-15 mmol/L Sodium dihydrogen phosphate（pH 9.2）. Results The linear ranges of adenosion, guanosion, uridine, inosion were in the concentration range of 10.1-252.5,10.3-258.0,10.3-257.6, and 10.8-270.0 mg/L, and r was 0.999 8, 0.999 7,0. 999 6, and 0. 999 5 respectively. The average recoveries were 98.4%, 102.5%, 97.2% and 101.3 %. RSD was 2.2 %, 1.8 %, 2.6 %, and 2.0 %, respectively. Conclusion The method is simple, and rapid, and it is suitable for the assay of nucleosions in Ningxinbao Capsules.

关 键 词：宁心宝胶囊 冬虫夏草 核苷类成分 毛细管电泳法 

分 类 号：R927.2[医药卫生—药学]