DNA-纳米金修饰玻碳电极用于水中甲醛的测定  

Electrical Detection of Formaldehyde in Aqueous Solution at a Glassy Carbon Electrode Modified with DNA and Nano-gold

作　　者：周忠亮[1] 郭秀锐[1] 鲁理平[1] 康天放[1] 程水源[1] 李珊曼[1] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100124

出　　处：《分析测试学报》2009年第6期697-700,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20247002);国家重点基础研究发展规划(973)项目(2005CB724201);北京市自然科学基金资助项目(8062010;8072004);北京工业大学校青基金资助项目(97005013200701)

摘　　要：利用纳米金的生物共容性和高电荷传递性能在玻碳电极表面构建纳米粒子生物活性界面，研究了DNA在其界面上对甲醛的电催化作用。通过电化学沉积方法制备了DNA-纳米金修饰的玻碳电极，并对该电极进行了形貌表征，发现平均直径为100nm的多面体纳米金均匀分布在电极表面。利用微分脉冲伏安法和安培法对甲醛进行了检测，优化了实验参数。结果表明：该修饰电极实现了对甲醛的灵敏测定，线性范围为1×10^-5～1×10^-3mol／L，检出限为1．0μmol／L。该电极表现出较好的稳定性，对实际水样的测定回收率为95％～103％.A biological activity interface was constructed by use of biocompatibility and good charge transfer properties of nano-gold on the glassy carbon electrode surface and the electroactivity of DNA for formaldehyde on the surface was investigated. A DNA -Au particles modified electrode was fabricated through electrochemical deposition method. The morphology of the electrode surface was characterized by atomic force microscopy and scan electron microscopy. The result indicated that a uniform polyhedral Au particles with average size of about 100 nm was deposited on the electrode surface. The electrochemical behavior of formaldehyde on modified electrode was investigated by cyclic vohammetric and differential pulse amperometric method. Experimental conditions were optimized. The resuits showed that the modified electrode exhibited a significant electrocatalytic activity toward formaldehyde. The oxidation peak current was linear with the concentration of formaldehyde in the range of 1×10^-5～1×10^-3mol/L with a detection limit of 1.0 μmol/L. The electrode showed good sensitivity and stability, and was applied in the determination of formaldehyde in real water samples with recoveries of 95% - 103% .

关 键 词：电化学生物传感器 甲醛 纳米金 脱氧核糖核酸(DNA) 

分 类 号：O657.15[理学—分析化学] TQ224.122[理学—化学]