外排系统与膜通透性的改变对淋球菌高水平多重耐药的影响  

Impact of functional enhancement of efflux pump system and reduced permeability of outer membrane on high-level multiple resistant Neisseria gonorrhoeae

作　　者：王冬梅[1] 王勇[1] 夏忠弟[2] 田峰 邹明祥[4] 

机构地区：[1]河南科技大学医学院微生物学教研室,河南洛阳471003 [2]中南大学湘雅医学院微生物学教研室,湖南长沙410008 [3]深圳沙河医院检验科,广东深圳518053 [4]中南大学湘雅医院检验科,湖南长沙410008

出　　处：《西安交通大学学报（医学版）》2009年第3期311-314,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：湖南省卫生厅科研基金资助项目(No.B2003-041)

摘　　要：目的探讨外排系统与膜通透性的改变对淋球菌多重耐药的影响。方法PCR扩增淋球菌mtrR启动子区域包含13 bp回文序列在内的157个碱基片段并进行DNA测序分析;提取外膜蛋白,利用SDS-PAGE进行组成型的分析。结果5株敏感株的mtrR启动子区域未发生突变,3株高水平多重耐药株的mtrR启动子区域13 bp回文序列均发生了单个A/T碱基的缺失,且均存在外膜孔蛋白表达的缺失。结论mtrR启动子区域13 bp回文序列单个A/T碱基的缺失引起的mtrCDE外排泵表达增加与外膜孔蛋白表达的缺失或下降导致的膜通透性降低,在介导淋球菌产生高水平多重耐药中起一定的作用。Objective To investigate the impact of functional enhancement of efflux pump system and reduced permeability of outer membrane on high-level multiple resistant Neisseria gonorrhocae. Methods Several highlevel multiple resistant isolates with erythromycin MIC= 128.0 rag/L, accompanied by concurrent resistance to several antimicrobial agents, were selected. 13 bp inverted sequencc positioned within the mtrR promoter region were amplified by PCR and directly sequenced to detect the possible gene change. The outer membrane proteins of the strains were extracted to analyze the constitutive profiles by SDS-PAGE. Results There were no gene mutations in 5 sensitive strains. All the 3 high-level multiple resistant strains contained the same mutation and cxhibited a single A/T base pair deletion in 13 bp inverted sequence positioned within the mtrR promoter region. Meanwhile porin protein 31 ku deficiency was found in all the 3 resistant strains. Conclusion The functional enhancement of efflux pump system induced by a single A/T base pair deletion in 13 bp inverted sequence positioned within the mtrR promoter region and the decreased cell envelope permeability induced by the absence of porin protein may have some effect on mediating high-level multiple resistance in Neisseria gonorrhoeae.

关 键 词：淋球菌 多重耐药 MTR 孔蛋白 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]